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  • 產品名稱:Nuclistain核酸染色劑(EB替代品)

  • 產品型號:EC-730
  • 產品廠商:其它品牌
  • 產品文檔:
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Nuclistain 核酸染色劑(EB替代品)
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Nuclistain核酸染色劑(EB替代品)  



  • UV Free Visualization of DNA and RNA
  • Improves Yield in PCR Purification from Agarose Gels
  • Easy and Safe
Catalog Number:EC-730

1.DNARNA檢測不需要紫外照射

2.提高PCR產物凝膠回收的完整性和產量

3.核酸陽性染色/高靈敏度

4.**便捷

Nuclistain核酸染色劑用來替代在單雙鏈DNA/RNA的瓊脂糖及聚丙烯酰胺電泳中使用的EB染色劑,它具有與EB相當的靈敏度,可以檢測低至10-50ng的核酸樣品。它的優勢在於正常的光照條件下可以檢測核酸,避免紫外照射對核酸的損傷。另外它的可見光檢測大幅度的提高了小於500bp的DNA樣品從瓊脂糖凝膠中的回收產量。Nuclistain核酸染色劑與核酸的結合是可逆的,不會對核酸進行修飾。Nuclistain核酸染色劑使用起來非常方便,一小瓶25ml的Nuclistain染色劑就可以稀釋成2.5L的染色工作液。分離的DNA條帶在淡藍色的背景下顯示為深藍色。

Nuclistain is intended as a replacement for the conventionally used ethidium bromide for the rapid detection of double and single stranded DNA and RNA in agarose and polyacrylamide gels. Its sensitivity is nearly comparable, with the capability of detecting as little as 10-50 ng of nucleic acid sample. The advantage of Nuclistain is that the results are visible under normal lighting conditions, eliminating the possibility of sample damage by UV radiation. Furthermore, Nuclistain visualization dramatically improves the yield of smaller and medium sized DNA (<500 bp) obtained by purification from agarose. Nuclistain binding is reversible and does not modify nucleic acids.

Nuclistain is extremely easy to use. One 25 ml bottle of Nuclistain will make 2.5 liters of stain solution. The separated DNA appears as dark blue bands on a light blue background.

 

Nuclistain improves the yield of smaller and medium sized DNA (<500 bp) obtained by purification from agarose. One gel was stained with Nuclistain and the other with EtBr. The DNA was subsequently purified from each and then run side by side on a third gel, visualized by EtBr. The results show the improved yield from the Nuclistain.

 

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