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  • 產品名稱：ProtoGlow ECL化學發光顯色（ECL）試劑盒

  • 產品型號：CL-300
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ProtoGlow ECL 化學發光顯色（ECL）試劑盒
詳情介紹：

ProtoGlow ECL化學發光顯色（ECL）試劑盒 


         增強化學發光法(ECL) Ecl 顯色
  • Enhanced chemiluminescent substrate
  • Extended signal life
  • Long shelf life/extra reliability
  • Less antibody needed
Catalog Number:CL-300

1.市場上*先進的加強型Western Blot化學發光底物

2.*長發光時間（120分鐘)，以利於優化性噪比

3.敏感性*高，需要更少的抗體

4.保質期長 (4攝氏度保存一年)

試劑盒采用的化學物質可以將WB的敏感度提高20倍.從而可以檢測到豐度更低的目的蛋白或者使用更高稀釋度的一抗二抗,從而大大的節約成本.試劑盒可以在120分鐘內保持穩定的發光, 從而保證每次曝光每張膠片都可以得到穩定的實驗結果.也可以從時間上允許多次曝光以選取合適的性噪比。可以檢測到5ng的目標蛋白，將抗體稀釋度提高4到40倍仍然可以得到良好的實驗結果,從而大大節約昂貴的抗血清成本.

National Diagnostics' ProtoGlow ECL delivers the latest technology developed for enhances chemiluminescent detection on Western blots. The unique chemistry of the ProtoGlow ECL system increases the sensitivity of Western blots by up to 20 fold. This allows the detection of proteins at lower abundance and/or the use of higher dilutions of primary and secondary antibodies, economizing on these expensive reagents.

The improved sensitivity of ProtoGlow ECL is demonstrated below. Serial dilutions of  transferrin were blocked and probed with HRP labelled antibody, and then subjected to detection using ProtoGlow ECL. ProtoGlow ECL detected down to 5ng of transferrin.

Gel detected with ProtoGlow ECL
Gel electroblotted onto nitrocellulose and probed with HRP-labelled antibody at 1:30,000 dilution. Detection using ProtoGlow ECL. Blots were exposed for 90 seconds. Lane 1) 100 ng; Lane 2) 50 ng; Lane 3) 25 ng; Lane 4) 10 ng; Lane 5) 5 ng.

 

Long Lasting Signal

ProtoGlow ECL generates a consistent light output for as long as 120 minutes. This ensures results are more reproducible: exposure to exposure, blot to blot. It also allows multiple exposures of a single blot to be taken to optimize the signal:noise ratio.

Gel detected with ProtoGlow ECL
Sequential exposures of serially diluted HRP conjugated anti-transferrin antibody detected with ProtoGlow ECL. Exposures of 60 seconds were taken at 0, 30, 60 and 180 minutes. Dilutions: Lane 1) 1:10,000; Lane 2) 1:20,000; Lane 3) 1:40,000; Lane 4) 1:80,000; Lane 5) 1:160,000.

Economical

ProtoGlow ECL allows the researcher to use less antibody. The extremely enhanced signal from ProtoGlow ECL allows you to use anywhere from 4 to 40 times less antibody per blot while retaining the same detection sensitivity. This in turn allows you to economize on the consumption of expensive antisera.


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