產品名稱：Nexterion Slide AL- Aldehydesilane Coated Nexterion Slide AL-醛矽烷塗層

Cleanroom cleaned and packaged

Sold in packs of 25 slides

Laser Barcoding available

Please inquire for volume pricing

Slide dimensions:75.6 x 25.0 x 1.0 mm

SKU: 1064874

Category: Coated Slides

潔淨室清潔和包裝

以25張幻燈片包裝出售

激光條形碼可用

請谘詢批量價格

滑動尺寸：75.6 x 25.0 x 1.0 mm

SKU：1064874

類彆：塗層幻燈片

Description

NEXTERION® Slide AL offers a good alternative to NEXTERION® Slide E (Epoxysilane slides) when an efficient covalent, and directed binding of amino-modified bio-molecules is required. NEXTERION® Slide AL is coated with an “active” slide chemistry coating that chemically binds bio-molecules to provide a high binding capacity, and minimize non-specific binding. The uniform surface features aldehyde groups that readily react with primary amines. Both 5’ or 3’ amine modified PCR products, and oligonucleotides work well with this surface chemistry. The covalent bond formed at the terminus of the nucleic acid offers both stability, and maximal base pairing opportunity. The covalent binding diminishes sample loss during the course of experiments, and permits more harsh wash steps, which reduce background, and allow for greater sensitivity. Additional immobilization steps, such as baking or UV cross-linking, are not required for immobilization. Furthermore, peptides, proteins (such as antibodies), cells, and tissues can be immobilized on NEXTERION® Slide AL via random binding to amine sites on the probes. The slides are easy to use, and are compatible with all commercially available arraying and scanning instruments.

Highly reproducible coating

NEXTERION® Slide AL are produced using an innovative process developed by SCHOTT to produce a consistent and reproducible coating. All slides are individually examined for physical defects and the presence of particles before and after coating. The density of the aldehyde groups on the coating remain constant over the entire surface of the slides, and has been optimized to provide the optimal binding capacity. The surface hydrophobicity is tightly controlled to optimize the performance with both contact and non-contact printers.

High intra-slide reproducibility of NEXTERION® Slide AL (20 k array of BSA labelled with Atto 547)

Packaging and storage

NEXTERION® Slide AL are packaged in chemically stable plastic boxes and sealed in an inert atmosphere. The slides are ready-to-use from the box, and are stable for 9 months in the sealed packaging when stored at room temperature.

描述

當需要有效的共價和定向結合氨基修飾的生物分子時，NEXTERION®SlideAL是NEXTERION®SlideE（環氧矽烷載玻片）的良好替代品。 NEXTERION®SlideAL塗有“活性”載玻片化學塗層，可以化學結合生物分子，提供高結合能力，並大限度地減少非特異性結合。均勻的表麵具有容易與伯胺反應的醛基。 5'或3'胺修飾的PCR產物和寡核苷酸均適用於該表麵化學。在核酸末端形成的共價鍵提供穩定性和大堿基配對機會。共價結合減少了實驗過程中的樣品損失，並允許更苛刻的洗滌步驟，這降低了背景，並允許更高的靈敏度。固定不需要額外的固定步驟，例如烘烤或UV交聯。此外，可以通過隨機結合探針上的胺位點將肽，蛋白質（如抗體），細胞和組織固定在NEXTERION®SlideAL上。這些載玻片易於使用，並且與所有市售的陣列和掃描儀器兼容。

高度可重複的塗層

NEXTERION®SlideAL采用肖特開發的工藝生產，可生成一致且可重複的塗層。在塗覆之前和之後，分彆檢查所有載玻片的物理缺陷和顆粒的存在。塗層上醛基的密度在載玻片的整個表麵上保持恒定，並且已經過優化以提供*佳的結合能力。嚴格控製表麵疏水性，以優化接觸式和非接觸式打印機的性能。

NEXTERION®SlideAL（20 k陣列BSA標記為Atto 547）的高載玻片內重現性

包裝和儲存

NEXTERION®SlideAL采用化學穩定的塑料盒包裝，密封在惰性氣氛中。載玻片可以從盒子中隨時使用，並且在室溫下儲存時可以在密封包裝中穩定9個月。