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  • 產品名稱：Hydrogen Peroxide Assay Kit過氧化氫檢測試劑盒

  • 產品型號：CL-204
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Hydrogen Peroxide Assay Kit 過氧化氫檢測試劑盒
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 Hydrogen Peroxide Assay Kit
過氧化氫檢測試劑盒 
   

  • Sensitive, Quantitative Assay for Hydrogen Peroxide
  • Detects as Little as 15ng/ml
  • Easy-to-use, Colorimetric System
Catalog Number:CL-204

 

1.靈敏的定量測定過氧化氫

2.*低檢測限為15ng/ml

3.采用比色方法，使用簡便

過氧化氫是氧還原途徑的產物，由分子氧雙電子還原或者*氧陰離子自由基單電子還原產生。過氧化氫為強氧化劑，其濃度水平必須準確檢測以完全確認被研究係統的氧化狀態。ND的過氧化氫檢測試劑盒提供了快速，靈敏，定量的檢測化學或生物係統中過氧化氫水平的方法。檢測方法基於二甲酚橙和三價鐵形成的複合物顯色反應，而三價鐵由過氧化物氧化二價鐵得到。反應可以比色定量化，過氧化物的*低檢測限為15ng/ml。該試劑盒是Diagenes過氧化物檢測試劑盒的補充，通過先後使用兩個試劑盒，研究者就可以定量檢測氧還原途徑的前兩步反應。

 


Hydrogen peroxide is a part of the oxygen reduction pathway, produced by the two-electron reduction of molecular oxygen, or by the one electron reduction of superoxide anion radical. Hydrogen peroxide is a potent oxidant, and the levels of hydrogen peroxide must be accurately determined in order to fully characterize the oxidative state of the system under study.

National Diagnostics Hydrogen Peroxide Assay Kit is a rapid, sensitive and quantitative method for the determination of Hydrogen Peroxide in chemical or biological systems. The assay is based upon formation of a complex between Xylenol Orange and ferric iron, which is produced by the peroxide dependent oxidation of ferrous iron. This reaction is quantified colorimetrically, detecting as little as 15ng/ml of peroxide.

National Diagnostics Hydrogen Peroxide Assay is the ideal complement to our well established Diogenes Superoxide detection system. Using these kits in tandem, researchers can now quantitatively determine the first two steps in the oxygen reduction pathway.


Hydrogen Peroxide Assay Protocol

Assay Reagent Preparation
Prepare 1.8ml of reagent per sample to run tests in duplicate.
1. To prepare 20ml: Combine 19.8ml of Component A with 0.2ml
Component B.
2. The mixture is stable at room temperature for one working day.

Assay Procedure
1. Mix 0.9ml of Assay Reagent with up to 0.1ml of sample.
2. Incubate at room temperature for at least 30 minutes to allow
for complete color development.
3. Read absorbance at 560nm.



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