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  • 產品名稱：Autofluor放射自顯影增強劑

  • 產品型號：LS-315
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簡單介紹：
Autofluor放射自顯影增強劑 比PPO-DMSO 效果好
詳情介紹：

 

Autofluor放射自顯影增強劑 

 
  • High resolution autoradiographic image intensifier
  • Rapid enhancement of low energy beta-emitters
  • For polyacrylamide gels, paper chromatography, and TLC plates
  • Water based, odorless, contains no DMSO
Catalog Number:LS-315

1.高分辨率的放射自顯影增強劑

2.迅速增強3H, 14C, 35S等低能量的β放射

3.適用於聚丙烯酰胺凝膠,紙層析和薄層層析

4.水性無味液體,不含DMSO

Autofluor放射自顯影增強劑**將水溶性閃爍熒光劑發展應用於放射自顯影增強方麵。它可以迅速滲入丙烯酰胺凝膠並將標記物傳遞給熒光劑的能量放大。它不含有DMSO也不含有任何芳香族的溶劑，避免了使用這些材料所帶來的危害。同時，使用非水溶性增強劑造成的條帶失真變形也不會存在。Autofluor放射自顯影增強劑是目前增強檢測β放射的所有方法中*簡單省時的一種。在八種獨立的聚丙烯酰胺凝膠中，35S標記蛋白的放射自顯影比較實驗，Autofluor是*有效的。使用Autofluor後半飽和x光片的dpm/mm2值隻需要單獨顯影的1/8。Autofluor應避免陽光直射，室溫下可以至少保存一年。避免冷凍，溫度低於20攝氏度時水溶性磷光劑可能會沉澱析出，加熱到30攝氏度可以使得磷光劑複溶。


Autofluor represents the first water soluble scintillation phosphor to be developed and applied directly for use as an autoradiographic image intensifier. Autofluor rapidly penetrates acrylamide gel systems and maximizes energy transfer from labeled compound to phosphor. Autofluor contains no dimethylsulfoxide or acidic aromatic solvents. Therefore, the hazards of use related to these materials are eliminated. The band distortion that is associated with using nonaqueous enhancers is also eliminated.

In the figure to the right, the gel on the left (7%, 1mm) was dehydrated in DMSO (dimethylsulfoxide) for one hour, then impregnated in PPO-DMSO for one hour and precipitated and dried. The gel on the right was impregnated with Autofluor for one hour and dried. Both gels were exposed for 24 hours at -76°C on Kodak XR-5 X-OMat film. The single tritiated band contains 5000 dpm. Note the higher degree of resolution and band discrimination with Autofluor vs PPO-DMSO.

The Autofluor procedure is the shortest and easiest procedure yet developed for enhancement and visualization of beta-emitters. In an independent test comparing eight different fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels, Autofluor was the most effective. With Autofluor, the dpm/mm2 required to half-saturate the x-ray film was 1/8 that required by autoradiography alone.
Storage: Autofluor has a shelf life of at least one year stored at room temperature, out of direct sunlight. Keep from freezing. At temperatures less than 20°C precipitation of water soluble phosphors may occur. Warming to approximately 30°C will redissolve the phosphors.


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