- 美國DRIERITE目錄-DRI...
- 美國Nationaldiagno...
- 美國Electron Micro...
-
美國電鏡耗材總目錄
-
腦矩陣
-
標樣
-
碳/石墨棒
- 清潔用品
-
鑽石刀
-
TEM支持膜
- 膠黏劑非導電 膠黏劑導電
-
燒杯
-
原子力顯微鏡用品
-
AFM探針
- 防靜電產品
- 膠黏劑產品
-
銅網、鎳網和金網
- Adhesives 膠粘劑 粘合...
-
Apertures 光圈孔徑
-
Atomic Force Mic...
-
Beakers 燒杯
-
Brushes 生物刷
-
Calibration 校準產品
-
Carbon / Graphit...
-
Cleaning 清潔產品
-
Dissection Suppl...
-
Filaments / Cath...
-
Embedding Suppli...
-
Forensic 法醫產品
-
FIB Supplies 纖維...
-
Grids 電鏡網格係列
-
Materials Scienc...
-
Omniprobe Consum...
-
Scanning Electro...
-
Tweezers 鑷子
-
Tools 工具
-
腦矩陣
-
KOVA公司產品
- 美國EIS
- 美國Tronex
-
APPLIED MICROARR...
- Drummondsci
- 美國PrimCells公司
- 美國Vector目錄-Vecto...
-
美國VWR目錄-VWR Cata...
-
General Catalogu...
- Adhesives-Autora...
- Bags-Barometers ...
- Baths-Blenders 水...
- Bottles 試驗瓶
- Brushes-Casserol...
- Centrifuges-Chlo...
- Chromatography 層...
- Clamps-Cylinders...
- Data Loggers-Ele...
- Electrophoresis ...
- Electroporators-...
- Filters-Flashlig...
- Flasks-Furnaces ...
- Gas-Heating Tape...
- Hemacytometers-H...
- Humidity-Ice Mak...
- Incubators-Melti...
- Microbiology Med...
- Microscopes-Mill...
- Molecular Biolog...
- Ovens-Petroleum ...
- pH-Photometers 酸...
- Pipets-Pipettors...
- Plates-Rulers 酶標...
- Safety, Air Moni...
- Safety, Hearing ...
- Sample Cups-Spec...
- Spectrophotomete...
- Standards 標準液
- Stationery-Stopp...
- Surface Tension-...
- Thermometers-Tim...
- Tissue-Tubes,NMR...
- Tubing-Valves 導管...
- Vials-Watch Glas...
- Water Purificati...
-
Chemical Catalog...
- Acacia to Alumin...
- Amine-Sulfuric A...
- Balsam Canada So...
- Cacodylic Acid, ...
- Deblock Reagent ...
- Ecolite to Furfu...
- D-(+)-Galactose ...
- Imidazole to Lys...
- Magnesium to Myr...
- Naphthalene to 2...
- Palladium (II) A...
- Potassium Acetat...
- Saccharin Sodium...
- Sorbic Acid to S...
- TAE Buffer to Tr...
- Universal Indica...
-
Life Science Cat...
- Air-Antibodies 壓...
- Apoptosis Reagen...
- Autoradiography-...
- Blotting-Bottles...
- Cell Cycle Reage...
- Centrifuges-Comb...
- Competent Cells ...
- Conductivity-Dia...
- DNA Synthesis Re...
- Electrophoresis-...
- Enzymes 酶
- Evaporators-Free...
- Fume Hoods-Histo...
- Homogenizers-Loo...
- Microscopes/Slid...
- Molecular Biolog...
- Nitric Oxide Rea...
- Peptide Synthesi...
- Petri-Photodocum...
- Pipetting 吸液管
- Plate Readers-Pl...
- Reagents, Specia...
- Refrigerators-Th...
- Tissue-Wipers 組織...
-
General Catalogu...
- 美國MatTek Corpora...
-
美國Nasco Whirl-Pa...
-
Whirl-Pak圓底采樣袋
-
Whirl-pak耐高溫采樣袋
-
Whirl-Pak大腸菌群采樣袋
-
Whirl-Pak均質袋
-
Whirl-Pak黑色采樣袋
-
Whirl-Pak無菌采樣袋(附...
-
Whirl-Pak可放置標牌的無...
-
Whirl-Pak防輻射采樣袋
-
Whirl-Pak帶泡沫采樣袋
-
Whirl-Pak長期保存樣品袋
-
Trans-Pak取樣袋,帶生物...
-
Whirl-Pak可標記寬鋼絲封...
-
Whirl-Pak直立含硫代硫酸...
-
Whirl-Pak大容積采樣袋
-
Whirl-Pak均質過濾袋
-
Whirl-Pak表麵測試采樣袋...
-
Whirl-Pak無菌水質采樣袋
-
Whirl-Pak可直立圓底采樣...
-
Whirl-Pak帶標簽無菌采樣...
-
Whirl-Pak標準無菌采樣袋
- Whirl-Pak®Bags 美...
-
Whirl-Pak圓底采樣袋
- Berkshire
- 美國CORNING目錄-CORN...
-
美國(Thermo Fisher...
-
**組化筆—PAP PEN
-
Thermo玻片-Thermo ...
- Fisher玻片-Fisher ...
- VWR玻片-VWR Slide
-
Corning玻片
-
NUNC玻片目錄- NUNC C...
-
紫外光譜相關-UV spectr...
-
HAMILTON進樣器-HAMI...
-
計時器-Timer
-
遊標式瓶口分配器-Cursor-...
-
AMPULES安瓿瓶-Ampou...
-
袋子-Bags
-
稱量用品-Weighing Pr...
-
燒杯-Beaker
-
實驗台保護墊-Test bed ...
-
試劑瓶-Reagent bott...
-
滴定管-Buret
-
遊標卡尺-Calipers
-
試劑桶-Reagent barr...
-
夾子和台架-Clip and S...
-
儲樣-Sample storag...
-
坩堝-Crucible
-
凍存-Cryopreservat...
-
量筒-Graduated cyl...
-
平皿-Plate
-
過濾產品-Filtration ...
-
燒瓶-Flask
-
容量瓶-Volumetric f...
-
地粘-stick
-
鑷子-Forceps
-
漏鬥-Funnel
-
玻璃割刀-Glass cutte...
-
手套-Gloves
-
組織學用具-Histology ...
-
冰盒-Ice box
-
標簽帶-Label with
-
微生物取樣-Microbial ...
-
微量板-Micro plate
-
顯微鏡耗材-Microscope...
-
瑪瑙研缽-Agate morta...
-
支架-Support
-
**用品-Safety Prod...
-
取樣器-Sampler
-
剪刀-Scissors
-
標準篩-Standard sie...
-
抹刀-Spatula
-
磁力攪拌子-Magnetic s...
-
膠塞-Stopper
-
組織培養-Tissue Cult...
-
夾子-Clip
-
分配盤/托盤-Distribut...
-
管子-Tube
-
樣品小瓶-Sample vial
-
利器存儲桶-Tool bucke...
-
實驗室用抹布-Laborator...
-
封口膜-Sealing film
-
移液管及**裝置-Pipette...
-
移液槍-Pipette
-
**組化筆—PAP PEN
- 美國BD 公司產品
- 美國Nalgene 生物耗材
- **新品-New Product...
- VWR目錄-VWR Catalo...
-
Corning目錄-Cornin...
-
Millipore儀器耗材-Mi...
-
Merck試劑耗材-Merck ...
-
Whatman試劑耗材-What...
-
Axygen試劑耗材-Axyge...
- SAI-infusion
- ASI-instruments
-
PALL儀器耗材-Pall Ca...
- 細胞目錄-Cell Lines
- 美國 Cell Marque
- Hamilton
- 菌株 菌種庫 微生物菌種
-
NU-CHEK
- ATCC細胞 細胞株 腫瘤細胞
- 丹麥NUNC全目錄
蛋白晶體取放工具MicroMounts™
MicroMounts™是晶體學(蛋白質晶體、病毒晶體、小分子、無機晶體等)實驗室常用顯微工具之一,也是傳統的和高通量的X射線晶體實驗中的理想操作工具,例如,可以用來從溶液中取出目標晶體移到X射線通路上。它的特殊材質和設計既可視且X光可透、具有一定的機械硬度、一定的柔韌度、精準的尺寸。
這些顯微工具的頭部由柔韌的微纖維聚酰亞胺薄膜製造,這種柔中帶剛的工具*易彎曲。這些工具可以測量樣品的範圍在毫米到幾個微米之間,與金屬工具相比,聚酰亞胺膜類工具對於很脆蛋白質晶體樣品可以達到*低的損傷度,且可視和X光可透。
每個聚酰亞胺膜頭部都鑲嵌在一個無磁不鏽鋼針座(pins)上,不鏽鋼針座的直徑大約0.64mm。針座再裝配到一直徑為0.7mm的手柄上,每個工具包都有手柄。該工具的應用領域:蛋白結晶學、單細胞以及較小樣品。
特性:
· 從同步加速器中或者其它小型射線源中方便快速的找到拿取50μm-100μm以下尺寸的晶體
· 從溶液中找到和拿取晶體
· **拿取的蛋白質尺寸
· 每次**放置在同一位置、X射線束下簡單排列
· *小化目標晶體周圍的液體,以得到*佳冷卻速率
· *小化背景信號使得X射線信號*大化
一、聚酰亞胺膜的模式:
1.晶體孔Crystal aperture,圖示A ,大小從10-600μm,孔邊緣的聚酰亞胺膜(圖中B)很窄,以小化各個方向上的散射背景。
2.毛細作用孔Wicking hole,圖中C,通過*細的導泄道drainage channel(圖中D)與晶體孔連接。孔的大小與15 or XF paper wicks相當。鋼筆樣式的設計可以很好的去除多餘的液體而不用the wick去接觸晶體。
3.十字孔Alignment cross,圖中E,位於從晶體孔中心對應的一個固定點上,便於晶體放置時的自動排列。
4.晶體孔大小代碼Crystal aperture size code,圖中F,圍繞十字孔分布,可以用來自動識彆孔的模式和晶體的大小。
5.晶體孔大小Aperture size,圖中G,微米大小,直接讀出。
6.方位標記孔Orientation mark ,圖中H,可以用來自動識彆樣品在正麵還是背麵。
聚酰亞胺膜參數如下表所示:
Material | Polyimide (used in Kapton tape) |
Crystal aperture sizes | 10, 20, 30, 50, 75, 100, 150, 200, 300, 400, 500 and 600 micrometers |
Thickness | 7.5, 10 and 12.5 micrometers |
Distance from center of alignment cross to center of crystal aperture | 870 micrometers |
Distance from pin base to center of crystal aperture | 12.1 mm (for 11 mm pins) |
Width of wicking hole | 250 micrometers. Compatible with Size 15 paper wicks. |
Length tolerance | 0.5 mm for 11, 19 and 25 mm pins 0.3 mm for SPINE pins. |
二、無磁不鏽鋼針座Solid 316 non-magnetic stainless steel pins.
提供的的針座有四種規格:11 mm, 19 mm, SPINE, and 25 mm,這裡的11,19,25mm與大家經常應用的尼龍環針座的10,18,24mm相對應的。
產品列表(**19mm和SPINE):
貨號 | 產品描述 | 貨號 | 產品描述 |
60115-S | MicroMounts, aperture 10µm, pin SPINE | 60115-19 | MicroMounts, aperture 10µm, pin 19mm |
60116-S | MicroMounts, aperture 20µm, pin SPINE | 60116-19 | MicroMounts, aperture 20µm, pin 19mm |
60117-S | MicroMounts, aperture 30µm, pin SPINE | 60117-19 | MicroMounts, aperture 30µm, pin 19mm |
60118-S | MicroMounts, aperture 50µm, pin SPINE | 60118-19 | MicroMounts, aperture 50µm, pin 19mm |
60119-S | MicroMounts, aperture 75µm, pin SPINE | 60119-19 | MicroMounts, aperture 75µm, pin 19mm |
60120-S | MicroMounts, aperture 100µm,pin SPINE | 60120-19 | MicroMounts, aperture 100µm, pin 19mm |
60121-S | MicroMounts, aperture 150µm,pin SPINE | 60121-19 | MicroMounts, aperture 150µm, pin 19mm |
60122-S | MicroMounts, aperture 200µm,pin SPINE | 60122-19 | MicroMounts, aperture 200µm, pin 19mm |
60123-S | MicroMounts, aperture 300µm,pin SPINE | 60123-19 | MicroMounts, aperture 300µm, pin 19mm |
60124-S | MicroMounts, aperture 400µm,pin SPINE | 60124-19 | MicroMounts, aperture 400µm, pin 19mm |
60125-S | MicroMounts, aperture 500µm,pin SPINE | 60125-19 | MicroMounts, aperture 500µm, pin 19mm |
60126-S | MicroMounts, aperture 600µm,pin SPINE | 60126-19 | MicroMounts, aperture 600µm, pin 19mm |
參考資料:
"Microfabricated mounts for high-throughput macromolecular cryocrystallography"
R. E. Thorne, Z. Stum, J. Kmetko, K. O'Neill and R. Gillilan
JOURNAL OF APPLIED CRYSTALLOGRAPHY
36: 1455-1460 December 2003
"A new crystal mounting method for macromolecular crystallography"
Z. Stum, J. Kmetko, K. O'Neill, R. L. Gillilan and R. E. Thorne
SYNCHROTRON RADIATION NEWS
17 (2): 31-32 April 2004