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*聲波振動鑽石刀Ultra Sonic Oscillating Diamond Knife
*聲波振動鑽石刀是室溫薄切片的選擇,也是近些年來DiATOME與Dr Daniel Studer,(Lab. of Anatomy, University of Berne)合作的結晶。*聲波振動切片是以*聲頻振動激勵鑽石刀以幫助改善切片性能方法,這種切片方法的研究,近年在美、日、蘇、西德、英等國取得較大進展並逐漸得到應用。其具有減少切力、提高加工精度、提高表麵光潔度和表麵質量、延長刀具壽命等。Ultra Sonic Oscillating Diamond Knife鑽石刀徹底解決了切片過程中的“樣本壓縮”問題。通常在切片過程中產生“樣本壓縮”的原因在於:
進刀的角度
樣品的硬度
鑽石表麵和樣品表麵的相互作用
切片的厚度
鑽石刀以固定的頻率和振幅來回運動,振蕩的方向要平行於刀鋒。在室溫下*聲振蕩鑽石刀製備的樣品
消除了壓縮的想象,讓使用者可以得到薄,質量好的切片。
我們已經進行以下包埋樣品的測試,其結果都非常理想:
生物學樣品in Epon, Araldite, EM Bed, etc
生物學樣品in acrylic resins (Lowicryls, LR White)
硬的聚合材料PS, PMMA, ABS, HIPS, modified PP, etc
該款鑽石刀的參數:
35º
刀鋒長3.0mm
切片厚度10-80nm
控製機單元 Control Unit
頻率25-45kHz,或者自動共振裝置
振幅可變(電壓0-30V)
機器電壓230V,110V
貨號 |
品名 |
角度 |
長度 |
30-ULTSON |
UltraSonic Knife and Control Unit |
35° |
3.0mm |
30-ULTSONK |
UltraSonic Knife |
35° |
3.0mm |
一些使用的參考文獻:
A. Al-Amoudi, J.Dubochet, H. Gnaegi, W. Lüthi, D.Studer: An oscillating cryo-knife reduces cuttinginduced deformation of vitreous ultrathin sections. Journal of Microscopy, Vol. 212, Pt 1, pp. 26-33, 2003.
D. Studer and H. Gnägi: Minimal compression of ultrathin sections with use of an oscillating diamond knife. Journal of Microscopy, Vol. 197, Pt 1, pp. 94-100, 2000.
J.S. Vastenhout and H.Gnaegi: Ultramicrotomy of polymers using an oscillating knife; improving polymer morphology. Microscopy and Microanalysis. 8 (Suppl. 2) 2002
使用此款鑽石刀得到的傑出作品展示如下:
Peripheral nerve (rat)
Peripheral nerv (rat), HP frozen, freeze substituted, Epon embedded, cut with the ultra sonic knife, section thickness 50nm. W. Graber, Institut of Anatomy, University of Bern, Switzerland
ABS, stained with OsO4, sectioned at room temperature with the ultra sonic knife, section thickness 50nm. Note the almost perfect spherical shape of the large rubber particles and the preservation of the inclusions inside. Also the smaller dense rubber particles are well preserved. B.Vastenhout, Dow Benelux N.V. Terneuzen, The Netherlands.
Heart (rat) |
Heart (rat), fixed with 2.5% Glutaraldehyde, OsO4 stained, Spurr's embedded, cut with the ultra sonic knife, section thickness setting 15nm. J. Nebesarova, Institut of Parasitology, Ceske Budejovice, Czech Republic. Imaged by Petr Stepan in LVEM 5 at 5kV, Delong Instruments, Brno, Czech Republic.