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  • 產品名稱：NR8383大鼠肺泡巨噬細胞

  • 產品型號：NR8383
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簡單介紹：
NR8383 (正常大鼠，1983年8月3日)來源於肺灌洗時的正常大鼠肺泡巨噬細胞。 細胞在gerbil肺細胞連續培養液存在下培養了大約8-9個月。 隨後，不再需要外源生長因子。通過有限稀釋法從單個細胞克隆並亞克隆NR8383細胞，並三次用軟瓊脂亞克隆。 細胞表現出巨噬細胞的特性，吞噬酵母多糖和銅綠，非特異性脂酶活性，Fc受體，氧化降解；分泌IL-1, TNF beta和IL-6，可重複地響應外源
詳情介紹：
細胞名稱
NR8383大鼠肺泡巨噬細胞
描述
NR8383 (正常大鼠，1983年8月3日)來源於肺灌洗時的正常大鼠肺泡巨噬細胞。 細胞在gerbil肺細胞連續培養液存在下培養了大約8-9個月。 隨後，不再需要外源生長因子。通過有限稀釋法從單個細胞克隆並亞克隆NR8383細胞，並三次用軟瓊脂亞克隆。 細胞表現出巨噬細胞的特性，吞噬酵母多糖和銅綠，非特異性脂酶活性，Fc受體，氧化降解；分泌IL-1, TNF beta和IL-6，可重複地響應外源生長因子。 NR8383細胞響應博萊黴素，分泌TGF beta前體。在博萊黴素刺激下，TGF beta mRNA表達也上升。 細胞對內**敏感。 1-10 鈉克/毫升的LPS水平抑製增生達50%。 即使達到0.001毫克/毫升的水平，LPS抑製還是無毒且在後續過程中可逆的。 NR8383細胞株提供了高響應的肺泡巨噬細胞的均一來源，可以用於體外研究巨響細胞相關活性。
動物種彆
大鼠
性彆
 
組織來源
肺；巨噬細胞；肺泡
形態
巨噬細胞
培養基和添加劑
F12K添加2mM L-穀氨酰胺， 1.5克/升碳酸氫鈉，及： 胎牛血清，20%。 這個配方適用於氣相中充入5%二氧化碳。
供應限製
A。僅供研究之用。
REFERENCE
Hidalgo HA , et al. Pneumocystis carinii induces an oxidative burst in alveolar macrophages. Infect. Immun. 60: 1-7, 1992. PubMed: 1729174 Helmke RJ , et al. A continuous alveolar macrophage cell line: comparisons with freshly derived alveolar macrophages. In Vitro Cell. Dev. Biol. 25: 44-48, 1989. PubMed: 2914814 Helmke RJ , et al. From growth factor dependence to growth factor responsiveness: the genesis of an alveolar macrophage cell line. In Vitro Cell. Dev. Biol. 23: 567-574, 1987. PubMed: 3497918 Limper AH , Standing JE . Vitronectin interacts with Candida albicans and augments organism attachment to the NR8383 macrophage cell line. Immunol. Lett. 42: 139-144, 1994. PubMed: 7534269 Hidalgo HA , et al. The effects of cyclosporine and dexamethasone on an alveolar macrophage cell line (NR8383). Transplantation 53: 620-623, 1992. PubMed: 1549855 Denholm EM , Rollins SM . Expression and secretion of transforming growth factor-beta by bleomycin-stimulated rat alveolar macrophages. Am. J. Physiol. 264: L36-L42, 1993. PubMed: 7679254 Krieg DP , et al. Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophages in vitro. Infect. Immun. 56: 3173-3169, 1988. PubMed: 3141284 Sherman MP , et al. Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages. Biochem. Biophys. Res. Commun. 191: 1301-1308, 1993. PubMed: 7682068 Griscavage JM , et al. Inducible nitric oxide synthase from a rat alveolar macrophage cell line is inhibited by nitric oxide. J. Immunol. 151: 6329-6337, 1993. PubMed: 7504017 Henderson SA , et al. Nitric oxide reduces early growth response-1 gene expression in rat lung macrophages treated with interferon-gamma and lipopolysaccharide. J. Biol. Chem. 269: 25239-25242, 1994. PubMed: 7523382 Huang S , et al. Rat KC cDNA cloning and mRNA expression in lung macrophages and fibroblasts. Biochem. Biophys. Res. Commun. 184: 922-929, 1992. PubMed: 1374243 Hay, R. J., Caputo, J. L, and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227. 1988. Fleming, D.O., Richardson, J. H., TuIis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.
 

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