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  • 產品名稱：NIH/3T3小鼠胚胎細胞

  • 產品型號：NIH/3T3
  • 產品廠商：其它品牌
  • 產品文檔：
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簡單介紹：
與原始的隨機交配3T3和近交係BALB/c 3T3建株方法一樣，NIH/3T3，是從NIH Swiss小鼠胚胎培養物中建立的高度接觸抑製的連續細胞株。 為了培育在形態學特征上更適合於進行轉化分析的亞株，建立的NIH/3T3細胞株又進行了五輪以上亞克隆。 這株細胞對DNA轉化及轉染研究十分有用。 檢測表明肢骨發育畸形病毒(鼠痘)陰性。
詳情介紹：
細胞名稱
NIH/3T3小鼠胚胎細胞
描述
與原始的隨機交配3T3和近交係BALB/c 3T3建株方法一樣，NIH/3T3，是從NIH Swiss小鼠胚胎培養物中建立的高度接觸抑製的連續細胞株。 為了培育在形態學特征上更適合於進行轉化分析的亞株，建立的NIH/3T3細胞株又進行了五輪以上亞克隆。 這株細胞對DNA轉化及轉染研究十分有用。 檢測表明肢骨發育畸形病毒(鼠痘)陰性。
動物種彆
小鼠
性彆
 
組織來源
胚胎
形態
成纖維細胞
培養基和添加劑
含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖，4mM L-穀氨酰胺的DMEM，90%；BCS，10%。
供應限製
A。僅供研究之用。
REFERENCE
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