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產品詳情
簡單介紹:
ND Protein Precipitation Kit
NDTM 蛋白沉澱試劑盒
詳情介紹:
ND Protein Precipitation Kit NDTM 蛋白沉澱試劑盒
- Recovery of All Proteins from Complex Mixtures
- Precipitates as Little as 100ng of BSA at 0.25µg/ml
- Milder than TCA
- More Reliable than Acetonitrile or Ammonium Sulfate
Catalog Number:EC-888
National Diagnostics公司的美國**ND蛋白沉澱試劑盒,具有以下優點:
1.從複雜的樣品中回收所有的蛋白
2.能夠從低至0.25µg/ml濃度的BSA蛋白樣品中回收100ng的微量蛋白
3.比TCA更加溫和
4.比乙腈和硫酸銨沉澱更加有效可靠
ND蛋白沉澱試劑盒沉澱過程溫和且易於操作。它可以保證在*低的蛋白濃度下*大量的回收溶液中的蛋白,其他方法中易丟失的蛋白也可以用該試劑盒沉澱回收。常規以及複雜的非常規的實驗都適用,真正做到了溫和性、簡便性和有效性。
The ND Protein Precipitation Kit is mild on samples and easy to use. It casts the finest net of any procedure, allowing the high yield collection of all proteins in solution, precipitating even the most dilute proteins. The ND Protein Precipitation Kit allows collection of proteins that would be missed by other methods.
Ideal for both routine and difficult work, the ND Protein Precipitation Kit stands alone among all methods. It offers the best combination of mildness, simplicity and effectiveness in protein precipitation.
High Yield Recovery
10ug BSA in 1ml vol and 50 ng of BSA in 200ul were prepared from stock and precipitated following kit instructions. 10ug and 50ng of BSA in 5 ul were prepared as controls. After running on a 12% gel, lanes 2 and 3 show control and recovered 10ug BSA. Lanes 5 and 6 show control and recovered 50ng BSA. Densitometry of 10ug BSA showed 91.9% recovery.
Simplicity of Method

Reagent A and Reagent B form a coprecipitate with the protein.

After a simple wash, the protein pellet is ready to be redissolved in desired buffer.