產品詳情
查看大圖

  • 產品名稱： CL-101 LLC-PK1 豬腎細胞

  • 產品型號： CL-101
  • 產品廠商：其它品牌
  • 產品文檔：
你添加了1件商品 查看購物車
簡單介紹：
簡單介紹 CL-101 LLC-PK1 豬腎細胞，原代細胞|細胞係|細胞株|菌種；細胞庫管理規範，提供的細胞株背景清楚，提供參考文獻和**培養條件！
詳情介紹：

CL-101 LLC-PK1 豬腎細胞 的詳細介紹
CL-101 LLC-PK1 豬腎細胞
ATCC® Number: CL-101™    Price:
Designations: LLC-PK1
Depositors:  Eli Lilly & Co.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
   
Organism: Sus scrofa (pig)
Morphology: epithelial
Source: Organ: kidney
Strain: Hampshire
Disease: normal
Cellular Products: plasminogen activator
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Age: 3 to 4 weeks
Gender: male
Propagation: ATCC complete growth medium: The base medium for this cell line is Medium 199 containing 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 3%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
formerly distributed as:ATCC CRL-1392
References: 3520: Hull RN, Huseby RM. Enhanced production of plasminogen activator. US Patent 3,904,480 dated Sep 9 1975
22659: Perantoni A, Berman JJ. Properties of Wilms' tumor line (TuWi) and pig kidney line (LLC-PK1) typical of normal kidney tubular epithelium. In Vitro 15: 446-454, 1979. PubMed: 225262
28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321


滬公網安備 31010902002433號