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  • 產品名稱：HB-191 PK136 小鼠X小鼠

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簡單介紹 HB-191 PK136 小鼠X小鼠，原代細胞|細胞係|細胞株|菌種；細胞庫管理規範，提供的細胞株背景清楚，提供參考文獻和**培養條件！
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HB-191 PK136 小鼠X小鼠
ATCC® Number: HB-191™    Price:
Designations: PK136
Depositors:  GC Koo
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse natural killer (NK) cells
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Comments: Animals were immunized with spleen and bone marrow cells from CE mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for NK cells and is cytotoxic in the presence of complement.
The pattern of reactivity of the antibody with cells of various strains of mice matches the pattern of conventional NK 1.1 antisera.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 5 X 10(4) and 1 X 10(6) cells/ml. Do not exceed 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 1319: Koo GC, et al. The NK-1.1 (-) mouse: a model to study differentiation of murine NK cells. J. Immunol. 137: 3742-3747, 1986. PubMed: 3782794
58145: Koo GC, Peppard JR. Establishment of monoclonal anti-NK-1.1 antibody. Hybridoma 3: 301-303, 1984. PubMed: 6500587


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