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  • 產品名稱： CTLL-2 小鼠T細胞 IL-2依賴型

  • 產品型號：TIB-214
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CTLL-2 小鼠T細胞 IL-2依賴型 的詳細介紹
CTLL-2  小鼠T細胞IL-2依賴型
ATCC® Number: TIB-214™    Price:
Designations: CTLL-2
Depositors:  S Gillis
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (mouse)
Morphology: lymphoblast
CTLL-2 小鼠T細胞 IL-2依賴型
Source: Cell Type: lymphocyte cytotoxic T lymphocyte;
Strain: C57BL/6
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Receptors: interleukin 2 (IL-2), expressed
Comments: This line is a clone of cytotoxic T cells derived from a C57BL/6 mouse. The cells are dependent upon IL-2 for growth, and can be used to assay for IL-2.Immediately after thawing (or after overgrowth of a culture) the culture will appear to have few or no viable cells. This is normal.Depending upon the source and potency of the IL-2, it may take up to three weeks before the cells are ready to subculture.T-STIM with Con A (rat IL-2 culture supplement from Becton Dickinson) may be used or the rat factor may be prepared as described on the product sheet. The freshly prepared rat factor will usually produce more rapid growth.Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: additional 2 mM L-glutamine; additional 1mM sodium pyruvate; adjust to a final concentration of 10% fetal bovine serum and 10% T-STIM with Con A. T-STIM is available from Becton Dickinson.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


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