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  • 產品名稱：HTB-48 SK-NEP-1 人腎母細胞瘤細胞

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簡單介紹 HTB-48 SK-NEP-1 人腎母細胞瘤細胞，原代細胞|細胞係|細胞株|菌種；細胞庫管理規範，提供的細胞株背景清楚，提供參考文獻和**培養條件！
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HTB-48 SK-NEP-1 人腎母細胞瘤細胞 的詳細介紹
HTB-48 SK-NEP-1 人腎母細胞瘤細胞
ATCC® Number: HTB-48™    Price:
Designations: SK-NEP-1
Depositors:  G Trempe, LJ Old
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: rounded
Source: Organ: kidney
Disease: Wilms' tumor
Derived from metastatic site: pleural effusion
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Tumorigenic: Yes
Antigen Expression: Blood Type A; Rh+
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,10
THO1: 8,9.3
TPOX: 8,11
vWA: 15,19
Cytogenetic Analysis: (P12) hypotriploid to hypertriploid (+A1, +A2, +C, +D, +E, +F, +G) with abnormalities including acrocentric fragments, secondary constrictions and large subtelocentric markers
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1-2
PGM3, 1
Age: 25 years
Gender: female
Ethnicity: Caucasian
Comments: Ultrastructural features include few microvilli, junctional complexes, well formed Golgi, mostly smooth ER, lipid droplets, no virus particles.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 X 10 exp5 cells/ml and maintain at between 10 exp5 and 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
26322: Orkin SH, et al. Development of homozygosity for chromosome 11p markers in Wilms' tumour. Nature 309: 172-174, 1984. PubMed: 6325937


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