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  • 產品名稱:CCL-185 A549 人肺癌細胞

  • 產品型號:CCL-185
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CCL-185 A549 人肺癌細胞 的詳細介紹
CCL-185 A549 人肺癌細胞
ATCC® Number: CCL-185™     Price:  
Designations: A549
Depositors:  M Lieber
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
CCL-185 A549 人肺癌細胞
Source: Organ: lung 
Disease: carcinoma
Cellular Products: keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Isolation: Isolation date: 1972
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X,Y 
CSF1PO: 10,12 
D13S317: 11 
D16S539: 11,12 
D5S818: 11 
D7S820: 8,11 
THO1: 8,9.3 
TPOX: 8,11 
vWA: 14
Cytogenetic Analysis: This is a hypotriploid human cell line with the modal chromosome number of 66, occurring in 24% of cells. Cells with 64 (22%), 65, and 67 chromosome counts also occurred at relatively high frequencies; the rate with higher ploidies was low at 0.4%. There were 6 markers present in single copies in all cells. They include der(6)t(1;6) (q11;q27); ?del(6) (p23); del(11) (q21), del(2) (q11), M4 and M5. Most cells had two X and two Y chromosomes. However, one or both Y chromosomes were lost in 40% of 50 cells analyzed. Chromosomes N2 and N6 had single copies per cell; and N12 and N17 usually had 4 copies.

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