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  • 產品名稱：CRL-3006 JeKo-1 人套細胞**瘤細胞

  • 產品型號：CRL-3006
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CRL-3006 JeKo-1 人套細胞**瘤細胞 的詳細介紹
CRL-3006 JeKo-1 人套細胞**瘤細胞
ATCC® Number: CRL-3006™     Price:  
Designations: JeKo-1
Depositors:  Elias Campo
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
CRL-3006 JeKo-1 人套細胞**瘤細胞
Characterization Data
Source: Organ: peripheral blood 
Tissue: Mantle Cell Lymphoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Antigen Expression: CD3-, CD5+, CD10+, CD19+ (verified at ATCC )
DNA Profile (STR): Amelogenin:X 
CSF1PO:9,12 
D13S317:8,9 
D16S539:12 
D5S818:10,13 
D7S820:10,11 
THO1:7 
TPOX:8 
vWA: 14
Age: 78
Gender: female
Comments: The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: 5% CO2 in air recommended 
Temperature: 37.0°C
Subculturing: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.
Preservation: Freeze medium: 30% RPMI; 60% FBS; 10% DMSO 
Storage temperature: liquid nitrogen vapor phase
References: 92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697 
16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063
ATCC® Number: CRL-3006™     Price: $417.00
Designations: JeKo-1
Depositors:  Elias Campo
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
CRL-3006 JeKo-1 人套細胞**瘤細胞
Characterization Data
Source: Organ: peripheral blood 
Tissue: Mantle Cell Lymphoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Antigen Expression: CD3-, CD5+, CD10+, CD19+ (verified at ATCC )
DNA Profile (STR): Amelogenin:X 
CSF1PO:9,12 
D13S317:8,9 
D16S539:12 
D5S818:10,13 
D7S820:10,11 
THO1:7 
TPOX:8 
vWA: 14
Age: 78
Gender: female
Comments: The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: 5% CO2 in air recommended 
Temperature: 37.0°C
Subculturing: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.
Preservation: Freeze medium: 30% RPMI; 60% FBS; 10% DMSO 
Storage temperature: liquid nitrogen vapor phase
References: 92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697 
16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063

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