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  • 產品名稱：MDA-MB-436 人乳腺腺癌細胞

  • 產品型號： HTB-130
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簡單介紹 MDA-MB-436 人乳腺腺癌細胞，原代細胞|細胞係|細胞株|菌種；細胞庫管理規範，提供的細胞株背景清楚，提供參考文獻和**培養條件！
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MDA-MB-436 人乳腺腺癌細胞 的詳細介紹
MDA-MB-436  人乳腺腺癌細胞
ATCC® Number: HTB-130™     Price:  
Designations: MDA-MB-436
Depositors:  R Cailleau
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: pleomorphic with multinucleated component cells
Source: Organ: mammary gland; breast 
Disease: adenocarcinoma 
Derived from metastatic site: pleural effusion
Cellular Products: tubulin; actin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Tumorigenic: No
DNA Profile (STR): Amelogenin: X 
CSF1PO: 12 
D13S317: 10 
D16S539: 9,11 
D5S818: 13 
D7S820: 10 
THO1: 9.3 
TPOX: 8 
vWA: 14,20
Cytogenetic Analysis: modal number = 45
Isoenzymes: AK-1, 1 
ES-D, 1 
G6PD, B 
GLO-I, 2 
Me-2, 1 
PGM1, 1 
PGM3, 1
Age: 43 years
Gender: female
Ethnicity: Caucasian
Comments: The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining.
Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended 
Medium Renewal: 2 to 3 times per week 
Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; DMSO, 5%
References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed:7000337
22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779
22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202
23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

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