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  • 產品名稱：CRL-1547 MNNG/HOS Cl #5 人骨肉瘤細胞

  • 產品型號：CRL-1547
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簡單介紹 CRL-1547 MNNG/HOS Cl #5 人骨肉瘤細胞，原代細胞|細胞係|細胞株|菌種；細胞庫管理規範，提供的細胞株背景清楚，提供參考文獻和**培養條件！
詳情介紹：

RL-1547 MNNG/HOS Cl #5 人骨肉瘤細胞
ATCC® Number: CRL-1547™     Price:  
Designations: MNNG/HOS Cl #5 [R-1059-D]
Depositors:  JS Rhim
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology:
Source: Organ: bone 
Disease: osteosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X 
CSF1PO: 12 
D13S317: 12 
D16S539: 10,13 
D5S818: 13 
D7S820: 11,12 
THO1: 6 
TPOX: 8,11 
vWA: 18
Isoenzymes: G6PD, B
Age: 13 years
Gender: female
Ethnicity: Caucasian
Comments: This line was derived from HOS (ATCC CRL-1543) by transformation with 0.01 mcg/ml MNNG (a carcinogenic nitrosamine). The cells exhibit a high saturation density, a high plating efficiency in soft agar and are tumorigenic in nude mice.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended 
Medium Renewal: 2 to 3 times per week 
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 2 to 5 days.
References: 22895: Rhim JS, et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046
26084: Rhim JS, et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298
32925: Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed:8650224

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