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  • 產品名稱：CCL-257 NCI-H1688 人經典小細胞肺癌細胞

  • 產品型號：CCL-257
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簡單介紹 CCL-257 NCI-H1688 人經典小細胞肺癌細胞，原代細胞|細胞係|細胞株|菌種；細胞庫管理規範，提供的細胞株背景清楚，提供參考文獻和**培養條件！
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CCL-257 NCI-H1688 人經典小細胞肺癌細胞 的詳細介紹
CCL-257 NCI-H1688 人經典小細胞肺癌細胞
ATCC® Number: CCL-257™     Price:  
Designations: NCI-H1688 [H1688]
Depositors:  AF Gazdar, JD Minna
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: lung 
Disease: carcinoma; classic small cell lung cancer
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
DNA Profile (STR): Amelogenin: X 
CSF1PO: 12 
D13S317: 8 
D16S539: 13 
D5S818: 10 
D7S820: 12 
THO1: 6,8 
TPOX: 11 
vWA: 17
Age: 50 years
Gender: male
Ethnicity: Caucasian
Comments: This line was derived from a liver metastasis taken from a patient prior to therapy.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended 
Medium Renewal: Every 2 to 3 days 
Remove spent medium, and add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes at room temperature. 
Remove the trypsin and incubate the flask at 37C for 5 to 10 minutes or until the cells detach. 
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020

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