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  • 產品名稱：CCL-155 RPMI 8226 人多發性骨髓瘤細胞

  • 產品型號：CCL-155
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簡單介紹 CCL-155 RPMI 8226 人多發性骨髓瘤細胞，ATCC 細胞|細胞係|細胞株|腫瘤細胞|細胞,細胞庫管理規範,提供 **培養條件,
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CCL-155 RPMI 8226 人多發性骨髓瘤細胞 的詳細介紹
CCL-155 RPMI 8226 人多發性骨髓瘤細胞
ATCC® Number: CCL-155™ 
Designations: RPMI 8226 
Isotype: lambda light chain 
Biosafety Level: 1 
Shipped: frozen 
Medium & Serum: See Propagation 
Growth Properties: suspension 
Organism: Homo sapiens (human) 
Morphology: lymphoblast


Source: Organ: peripheral blood 
Disease: plasmacytoma; myeloma 
Cell Type: B lymphocyte; 
Cellular Products: immunoglobulin light chain 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 

Isolation: Isolation date: June, 1966 
Applications: transfection host 
Virus Susceptibility: Human poliovirus 1
Herpes simplex virus
Vaccinia virus
Vesicular stomatitis virus 
Antigen Expression: HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e + 
DNA Profile (STR): Amelogenin: X,Y 
CSF1PO: 12 
D13S317: 11 
D16S539: 9 
D5S818: 11,13 
D7S820: 9,10 
THO1: 8 
TPOX: 8,11 
vWA: 16,18 
Cytogenetic Analysis: Unstable karyotype in triploid range of 68-70 chromosomes. Two large marker chromosomes with terminal centromeres. 
Isoenzymes: G6PD, A 
Age: 61 years 
Gender: male 
Comments: There is no evidence of heavy chain production (cytoplasmic or secreted). 
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C 
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.Do not allow the cell density to exceed 2 to 3 X 10(6) cells/ml. 
Interval: Maintain cell density between 5 X 10(5) and 2 X 10(6) viable cells/ml. 
Medium Renewal: Every 2 to 3 days 
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
purified DNA:ATCC CCL-155D 
References: 1030: Matsuoka Y, et al. Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma. Proc. Soc. Exp. Biol. Med. 125: 1246-1250, 1967. PubMed: 6042436
26017: Moore GE, Kitamura H. Cell line derived from patient with myeloma. N.Y. State J. Med. 68: 2054-2060, 1968. PubMed: 5243066
43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375 

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