產品詳情
  • 產品名稱:環氧化物包被微陣列芯片

  • 產品型號:40040
  • 產品廠商:其它品牌
  • 產品文檔:
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簡單介紹:
Corning® Epoxide Coated Slides are designed for covalent attachment of oligonucleotides (both short and long oligos, with and without amino-modification). The Corning® Epoxide Slide Starter Kit offe
詳情介紹:
環氧化物包被微陣列芯片
穩定的表麵可共價結合短寡聚核苷酸(有氨基修飾或者無氨基修飾,~30-mer)、長寡聚核苷酸(~>50-mer)和cDNA。配合Pronto!TM 通用雜交試劑盒可使芯片整體性能達到zui高。
特點
多功能性: 對短寡聚核苷酸、長寡聚核苷酸和cDNA具有同樣效果。可印跡有氨基修飾或無氨基修飾的寡聚核苷酸。DNA耦合無需UV交聯或烘烤步驟。
重複性:玻片間差異*小,不到3%CV
靈敏度:在4μg總RNA中可檢測1pg RNA
特異性:可區分同源性寡聚核苷酸90%(30-mer寡聚核苷酸中有3個錯配)


Corning® Epoxide Coated Slides are designed for covalent attachment of oligonucleotides (both short and long oligos, with and without amino-modification).


The Corning® Epoxide Slide Starter Kit offers 10 Epoxide Coated Slides, 0.8 mL of Pronto!™ Long-Oligo/cDNA Hybridization Solution, and 0.8 mL Pronto!™ Short Oligo Hybridization Solution. This kit is being offered as a way of providing a low cost trial of critical components for the optimization of oligo-array production and the subsequent hybridization to labeled cDNA.

The Corning® Epoxide Slides work best when oligos are dissolved in 150 mM sodium phosphate, ph 8.5 to 9.0. A small amount of detergent may be added to increase spot size, depending on printing conditions and the type of pins used.

When Corning® Epoxide Coated Slides are used in conjunction with the Pronto!™ Universal Hybridization Kit, the following features are evident:
• Sensitivity to 1 pg target RNA in a 4 µg total RNA direct labeling reaction
• Interslide CVs below 10%
• Detection of low expression targets above extremely low backgrounds



product details at-a-glance
Material: Glass
Qty./Pk.: 1
Qty./CS.: 1
Barcoded: Yes
Dimensions (LxWxH): 25.3x75.5x1.1 mm (approx)
Sterile: No
Coating: Epoxide

product details

10 Epoxide Coated Slides, 0.8mL Short Oligo and 0.8mL cDNA/Long Oligo Hybridization Solution

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