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抗熒光衰退封片劑
1.抗熒光衰退封片劑(CITIFLUOR™ - Antifadent Mountant Solutions)
適於熒光封片。由於生物樣品的多樣性以及**熒光組化中熒光衰退的複雜性,並冇有一個完全通用的抗熒光衰退劑,常見的抗熒光衰退劑有AF1、AF2、AF3、AF87,其中AF87是一個**的油性抗熒光衰退劑。
保存溫度 |
貨號 |
品名 |
規格 |
RT |
17970-25 |
CitiFluor™ AF1 |
25 ml |
RT |
17971-25 |
CitiFluor™ AF2 |
25 ml |
RT |
17972-25 |
CitiFluor™ AF3 |
25 ml |
RT |
17976-10 |
CitiFluor™ AF87 |
10 ml |
RT |
17978-35 |
Set of CitiFluor™ CFPVOH and AF100* |
25 ml+ 5 ml |
產品選購指南:
There is no universal antifadent due to the fact that biological material is so varied in nature that the fluorescence fading of fluorochromic conjugates is a complex process.
The general anti-fadents are AF1, AF2, and AF3. The AF87 is a specialist immersion oil-based antifadent.
AF1 (Glycerol-phosphate buffered solution containing an additive for use with labeled tissue sections). Should be used if you normally examine specimens in glycol solution. If you normally use saline solution with your specimens.
AF2 (Glycerol solution containing additives for use with labeled tissue sections) is simply a glycerol solution of the antifadent.
AF3 (Phosphate-buffered saline solution containing additives for examination of whole cell) is appropriate.
AF87, the antifadent, is contained in non-fluorescent immersion oil. It is designed for those requiring work at high magnification. It is useful for preventing the fading of such dyes as DAPI, which have the tendency to disperse in glycerol based mountant solutions.
CFPVOH is an aqueous solution of poly(vinyl alcohol) for use as a solid CFPVOH is an aqueous solution of poly (vinyl alcohol) for use as a solid mountant. Apply a few drops of the solution to your sample, and allow drying to give a clear film.
AF100 is a solution of antifadents to be used with CFPVOH when fading of fluorescence in the poly(vinyl alcohol) is a problem. To prepare a CFPVOH solution containing AF100, add 0.1ml of Af100 to 0.9ml of CFPVOH. If more or less of the solid mountant solution is required, mix the reagent in the appropriate ratio. Once these solutions have been made up, they should be used within a period of days since the solution deteriorates on storage.
All of these products have a long shelf life, a minimum of 2 years. The solution can be stored at room temp-erature. With LD50’s >2000mg/kg (in rat), this product exhibits no toxicological problems.