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NU-CHEK
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Cleanroom cleaned and packaged
Sold in packs of 25 slides
Laser Barcoding available
Please inquire for volume pricing
Slide dimensions:75.6 x 25.0 x 1.0 mm
SKU: 1064874
Category: Coated Slides
洁净室清洁和包装
以25张幻灯片包装出售
激光条形码可用
请咨询批量价格
滑动尺寸:75.6 x 25.0 x 1.0 mm
SKU:1064874
类别:涂层幻灯片
Description
NEXTERION® Slide AL offers a good alternative to NEXTERION® Slide E (Epoxysilane slides) when an efficient covalent, and directed binding of amino-modified bio-molecules is required. NEXTERION® Slide AL is coated with an “active” slide chemistry coating that chemically binds bio-molecules to provide a high binding capacity, and minimize non-specific binding. The uniform surface features aldehyde groups that readily react with primary amines. Both 5’ or 3’ amine modified PCR products, and oligonucleotides work well with this surface chemistry. The covalent bond formed at the terminus of the nucleic acid offers both stability, and maximal base pairing opportunity. The covalent binding diminishes sample loss during the course of experiments, and permits more harsh wash steps, which reduce background, and allow for greater sensitivity. Additional immobilization steps, such as baking or UV cross-linking, are not required for immobilization. Furthermore, peptides, proteins (such as antibodies), cells, and tissues can be immobilized on NEXTERION® Slide AL via random binding to amine sites on the probes. The slides are easy to use, and are compatible with all commercially available arraying and scanning instruments.
Highly reproducible coating
NEXTERION® Slide AL are produced using an innovative process developed by SCHOTT to produce a consistent and reproducible coating. All slides are individually examined for physical defects and the presence of particles before and after coating. The density of the aldehyde groups on the coating remain constant over the entire surface of the slides, and has been optimized to provide the optimal binding capacity. The surface hydrophobicity is tightly controlled to optimize the performance with both contact and non-contact printers.
High intra-slide reproducibility of NEXTERION® Slide AL (20 k array of BSA labelled with Atto 547)
Packaging and storage
NEXTERION® Slide AL are packaged in chemically stable plastic boxes and sealed in an inert atmosphere. The slides are ready-to-use from the box, and are stable for 9 months in the sealed packaging when stored at room temperature.
描述
当需要有效的共价和定向结合氨基修饰的生物分子时,NEXTERION®SlideAL是NEXTERION®SlideE(环氧硅烷载玻片)的良好替代品。 NEXTERION®SlideAL涂有“活性”载玻片化学涂层,可以化学结合生物分子,提供高结合能力,并大限度地减少非特异性结合。均匀的表面具有容易与伯胺反应的醛基。 5'或3'胺修饰的PCR产物和寡核苷酸均适用于该表面化学。在核酸末端形成的共价键提供稳定性和大碱基配对机会。共价结合减少了实验过程中的样品损失,并允许更苛刻的洗涤步骤,这降低了背景,并允许更高的灵敏度。固定不需要额外的固定步骤,例如烘烤或UV交联。此外,可以通过随机结合探针上的胺位点将肽,蛋白质(如抗体),细胞和组织固定在NEXTERION®SlideAL上。这些载玻片易于使用,并且与所有市售的阵列和扫描仪器兼容。
高度可重复的涂层
NEXTERION®SlideAL采用肖特开发的工艺生产,可生成一致且可重复的涂层。在涂覆之前和之后,分别检查所有载玻片的物理缺陷和颗粒的存在。涂层上醛基的密度在载玻片的整个表面上保持恒定,并且已经过优化以提供*佳的结合能力。严格控制表面疏水性,以优化接触式和非接触式打印机的性能。
NEXTERION®SlideAL(20 k阵列BSA标记为Atto 547)的高载玻片内重现性
包装和储存
NEXTERION®SlideAL采用化学稳定的塑料盒包装,密封在惰性气氛中。载玻片可以从盒子中随时使用,并且在室温下储存时可以在密封包装中稳定9个月。