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  • 产品名称：CRL-1708 QT6 鹌鹑纤维肉瘤细胞

  • 产品型号：CRL-1708
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简单介绍 CRL-1708 QT6 鹌鹑纤维肉瘤细胞，ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
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CRL-1708 QT6 鹌鹑纤维肉瘤细胞
ATCC® Number: CRL-1708™    Price:
Designations: QT6
Depositors:  C Moscovici
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Coturnix coturnix japonica (quail, Japanese)
Morphology: fibroblast
Source: Disease: fibrosarcoma
Cell Type: methylcholanthrene induced
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Virus Susceptibility: Rous sarcoma virus
Avian encephalomyelitis virus
Rabies virus
Marek's disease virus
Tumorigenic: Yes
Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
Temperature: 37.0°C
Subculturing: Protocol: Resuspend cells by pipetting fresh medium across the monolayer. Cells may also be dislodged by hitting culture flask against the palm of the hand. Subculture at approximately 75% confluency. Use of 0.5% polyvinylpyrrolidone (PVP) in atrypsin-EDTA solution increases the viability when subculturing by enzymatic means. Neutralize and centrifuge to remove additive.Note: When subcultures are prepared using pipetting or mechanical means thecells adhere lightly to the flask substrate and exhibit a tendency toward clumping.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2030
References: 22385: Moscovici C, et al. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11: 95-103, 1977. PubMed: 194709


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