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  • 产品名称：CRL-10762 CTLA4 Ig-24 中国仓鼠卵巢细胞

  • 产品型号：CRL-10762
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简单介绍：
简单介绍 CRL-10762 CTLA4 Ig-24 中国仓鼠卵巢细胞，ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
详情介绍：

ATCC® Number: CRL-10762™    Price:  
Designations: CTLA4 Ig-24
Depositors:  Bristol-Myers Squibb Co.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cricetulus griseus (hamster, Chinese)
Morphology: CRL-10762 CTLA4 Ig-24 中国仓鼠卵巢细胞epithelial
Source: Organ: ovary
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Gender: female
Comments: The line was constructed by transfecting CHO cells with a construct (CTLA4Ig) containing the sequence for the extracellular domain of the human CTLA-4 gene fused to the sequence for the hinge, CH2 and CH3 regions of human IgC.gamma.1 domain.The cells express the fusion protein (CTLA4Ig).
The cells express the fusion protein (CTLA4Ig).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 0.2 mM proline, 0.001 mM methotrexate, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
References: 4548: Linsley PS. Chimeric CTLA4 receptor and methods for its use. US Patent 5,434,131 dated Jul 18 1995


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