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低粘度包埋剂试剂盒(Spurr树脂包埋试剂盒)
低粘度包埋剂试剂盒(Spurr树脂包埋试剂盒)常用于包埋生物组织块,该试剂具有极好的渗透性能 。低粘度包埋剂试剂盒(Spurr树脂包埋试剂盒)使用简单,配制快速方便,而且包埋剂的硬度可以通过改变DER736的加入比例进行调节。使用该试剂盒得到的包埋块具有很好的外观,易于切片,在电子束下超薄切片也具有很好的硬度。
试剂盒内容物:
NSA 450ml
ERL4221 225ml
DER736 225ml
DMAE 25ml
配产品选购:
货号 | 产品名称 | 规格 |
14300 | LOW VISCOSITY EMBEDDING KIT(Spurr embedding kit) | Kit |
制方法指南:
Add each component in turn to a disposable plastic beaker. An exact weight is recommended, and care must be used in dispensing the final amounts of each component so that no excess is added.
The catalyst (DMAE) should be added last, after gently mixing the three other components.
The complete formula should be mixed thoroughly.
The complete mixture with the hardener can be used immediately for infiltration, and then for embedding. Although the mixture can be stored in a disposable syringe, well capped and with no air, in a freezer for several months it is highly recommended that freshly prepared embedding medium always be used. If you choose to store the mixture, it is imperative that you warm it thoroughly prior to use.
配制组分参考:
项目 | Firm Standard | Hard | Soft | Longer Pot Life |
ERL 4221 | 10.0g | 10.0g | 10.0g | 10.0g |
DER 736 | 6.0g | 4.0g | 8.0g | 6.0g |
NSA | 26.0g | 26.0g | 26.0g | 26.0g |
DMAE | 0.3g | 0.3g | 0.3g | 0.2g |
Cure Time at 70°C | 8 hours | 8 hours | 8 hours | 16 hours |
Pot Life | 3-4 days | 3-4 days | 3-4 days | 7 days |
小知识:请参阅包埋剂的选择 http://www.sangerbio.com/s04/sangerbio/jswz/20100406/292062.html