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  • 产品名称：CRL-8027 OKT 11杂交瘤细胞抗CD2

  • 产品型号：CRL-8027
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CRL-8027 OKT 11杂交瘤细胞抗CD2 的详细介绍
CRL-8027 OKT 11杂交瘤细胞抗CD2
ATCC® Number: CRL-8027™    Price:
Designations: OKT 11
Depositors:  Ortho Pharmaceutical Corporation
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human T cell (human T lymphocyte) and human E rosette positive thymocytes (human thymic lymphocyte); against CD2
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Comments: Mice were immunized with human T cell leukemia cells (acute lymphoblastic leukemia).
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3507: Kung PC, Goldstein G. Monoclonal antibody to a human T cell antigen and methods of preparing same. US Patent 4,364,937 dated Dec 27 1982


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