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  • 产品名称： CRL-6475 B16F10 小鼠黑色素瘤高转移细胞

  • 产品型号： CRL-6475
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简单介绍 CRL-6475 B16F10 小鼠黑色素瘤高转移细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件!
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CRL-6475 B16F10 小鼠黑色素瘤高转移细胞 的详细介绍
CRL-6475 B16F10 小鼠黑色素瘤高转移细胞
ATCC® Number: CRL-6475™    Price:
Designations: B16-F10
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: melanocyte
CRL-6475 B16F10 小鼠黑色素瘤高转移细胞
Source: Organ: skin
Strain: C57BL/6J
Disease: melanoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (technology from amaxa)
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

      Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended
      Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


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