产品名称：CCL-219 L1210 小鼠白血病细胞

CCL-219 L1210 小鼠白血病细胞
ATCC® Number:  CCL-219™     
Designations:  L1210 
Depositors:   EM Jensen 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: suspension
Organism: Mus musculus (mouse) 
Morphology: lymphoblast

 
Source: Disease: lymphocytic leukemia
Strain: DBA subline 212
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Tumorigenic: Yes 
Reverse Transcript: positive 
Cytogenetic Analysis: modal number = 40; range = 32 to 80.
Mouse karyotype with a diploid stemline number of 40 chromosomes.
Age:  8 months 
Gender:  female 
Comments: Tested and found negative for ectromelia virus (mousepox).
The line was established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether. [22513]
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:  Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 viable cells/ml.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 4 days
Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time:  8 to 10 hrs 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2040
References: 22513: . . J. Natl. Cancer Inst. 10: 179-192, 1949.
26055: . . J. Natl. Cancer Inst. 36: 405-421, 1966.
26056: . . Cancer Chemother. Rep. 51: 451-453, 1967.
32923: Young SW, et al. Gadolinium(III) texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865
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