- 美国DRIERITE目录-DRI...
- 美国Nationaldiagno...
- 美国Electron Micro...
-
美国电镜耗材总目录
-
脑矩阵
-
标样
-
碳/石墨棒
- 清洁用品
-
钻石刀
-
TEM支持膜
- 胶黏剂非导电 胶黏剂导电
-
烧杯
-
原子力显微镜用品
-
AFM探针
- 防静电产品
- 胶黏剂产品
-
铜网、镍网和金网
- Adhesives 胶粘剂 粘合...
-
Apertures 光圈孔径
-
Atomic Force Mic...
-
Beakers 烧杯
-
Brushes 生物刷
-
Calibration 校准产品
-
Carbon / Graphit...
-
Cleaning 清洁产品
-
Dissection Suppl...
-
Filaments / Cath...
-
Embedding Suppli...
-
Forensic 法医产品
-
FIB Supplies 纤维...
-
Grids 电镜网格系列
-
Materials Scienc...
-
Omniprobe Consum...
-
Scanning Electro...
-
Tweezers 镊子
-
Tools 工具
-
脑矩阵
-
KOVA公司产品
- 美国EIS
- 美国Tronex
-
APPLIED MICROARR...
- Drummondsci
- 美国PrimCells公司
- 美国Vector目录-Vecto...
-
美国VWR目录-VWR Cata...
-
General Catalogu...
- Adhesives-Autora...
- Bags-Barometers ...
- Baths-Blenders 水...
- Bottles 试验瓶
- Brushes-Casserol...
- Centrifuges-Chlo...
- Chromatography 层...
- Clamps-Cylinders...
- Data Loggers-Ele...
- Electrophoresis ...
- Electroporators-...
- Filters-Flashlig...
- Flasks-Furnaces ...
- Gas-Heating Tape...
- Hemacytometers-H...
- Humidity-Ice Mak...
- Incubators-Melti...
- Microbiology Med...
- Microscopes-Mill...
- Molecular Biolog...
- Ovens-Petroleum ...
- pH-Photometers 酸...
- Pipets-Pipettors...
- Plates-Rulers 酶标...
- Safety, Air Moni...
- Safety, Hearing ...
- Sample Cups-Spec...
- Spectrophotomete...
- Standards 标准液
- Stationery-Stopp...
- Surface Tension-...
- Thermometers-Tim...
- Tissue-Tubes,NMR...
- Tubing-Valves 导管...
- Vials-Watch Glas...
- Water Purificati...
-
Chemical Catalog...
- Acacia to Alumin...
- Amine-Sulfuric A...
- Balsam Canada So...
- Cacodylic Acid, ...
- Deblock Reagent ...
- Ecolite to Furfu...
- D-(+)-Galactose ...
- Imidazole to Lys...
- Magnesium to Myr...
- Naphthalene to 2...
- Palladium (II) A...
- Potassium Acetat...
- Saccharin Sodium...
- Sorbic Acid to S...
- TAE Buffer to Tr...
- Universal Indica...
-
Life Science Cat...
- Air-Antibodies 压...
- Apoptosis Reagen...
- Autoradiography-...
- Blotting-Bottles...
- Cell Cycle Reage...
- Centrifuges-Comb...
- Competent Cells ...
- Conductivity-Dia...
- DNA Synthesis Re...
- Electrophoresis-...
- Enzymes 酶
- Evaporators-Free...
- Fume Hoods-Histo...
- Homogenizers-Loo...
- Microscopes/Slid...
- Molecular Biolog...
- Nitric Oxide Rea...
- Peptide Synthesi...
- Petri-Photodocum...
- Pipetting 吸液管
- Plate Readers-Pl...
- Reagents, Specia...
- Refrigerators-Th...
- Tissue-Wipers 组织...
-
General Catalogu...
- 美国MatTek Corpora...
-
美国Nasco Whirl-Pa...
-
Whirl-Pak圆底采样袋
-
Whirl-pak耐高温采样袋
-
Whirl-Pak大肠菌群采样袋
-
Whirl-Pak均质袋
-
Whirl-Pak黑色采样袋
-
Whirl-Pak无菌采样袋(附...
-
Whirl-Pak可放置标牌的无...
-
Whirl-Pak防辐射采样袋
-
Whirl-Pak带泡沫采样袋
-
Whirl-Pak长期保存样品袋
-
Trans-Pak取样袋,带生物...
-
Whirl-Pak可标记宽钢丝封...
-
Whirl-Pak直立含硫代硫酸...
-
Whirl-Pak大容积采样袋
-
Whirl-Pak均质过滤袋
-
Whirl-Pak表面测试采样袋...
-
Whirl-Pak无菌水质采样袋
-
Whirl-Pak可直立圆底采样...
-
Whirl-Pak带标签无菌采样...
-
Whirl-Pak标准无菌采样袋
- Whirl-Pak®Bags 美...
-
Whirl-Pak圆底采样袋
- Berkshire
- 美国CORNING目录-CORN...
-
美国(Thermo Fisher...
-
**组化笔—PAP PEN
-
Thermo玻片-Thermo ...
- Fisher玻片-Fisher ...
- VWR玻片-VWR Slide
-
Corning玻片
-
NUNC玻片目录- NUNC C...
-
紫外光谱相关-UV spectr...
-
HAMILTON进样器-HAMI...
-
计时器-Timer
-
游标式瓶口分配器-Cursor-...
-
AMPULES安瓿瓶-Ampou...
-
袋子-Bags
-
称量用品-Weighing Pr...
-
烧杯-Beaker
-
实验台保护垫-Test bed ...
-
试剂瓶-Reagent bott...
-
滴定管-Buret
-
游标卡尺-Calipers
-
试剂桶-Reagent barr...
-
夹子和台架-Clip and S...
-
储样-Sample storag...
-
坩埚-Crucible
-
冻存-Cryopreservat...
-
量筒-Graduated cyl...
-
平皿-Plate
-
过滤产品-Filtration ...
-
烧瓶-Flask
-
容量瓶-Volumetric f...
-
地粘-stick
-
镊子-Forceps
-
漏斗-Funnel
-
玻璃割刀-Glass cutte...
-
手套-Gloves
-
组织学用具-Histology ...
-
冰盒-Ice box
-
标签带-Label with
-
微生物取样-Microbial ...
-
微量板-Micro plate
-
显微镜耗材-Microscope...
-
玛瑙研钵-Agate morta...
-
支架-Support
-
**用品-Safety Prod...
-
取样器-Sampler
-
剪刀-Scissors
-
标准筛-Standard sie...
-
抹刀-Spatula
-
磁力搅拌子-Magnetic s...
-
胶塞-Stopper
-
组织培养-Tissue Cult...
-
夹子-Clip
-
分配盘/托盘-Distribut...
-
管子-Tube
-
样品小瓶-Sample vial
-
利器存储桶-Tool bucke...
-
实验室用抹布-Laborator...
-
封口膜-Sealing film
-
移液管及**装置-Pipette...
-
移液枪-Pipette
-
**组化笔—PAP PEN
- 美国BD 公司产品
- 美国Nalgene 生物耗材
- 推荐新品-New Product...
- VWR目录-VWR Catalo...
-
Corning目录-Cornin...
-
Millipore仪器耗材-Mi...
-
Merck试剂耗材-Merck ...
-
Whatman试剂耗材-What...
-
Axygen试剂耗材-Axyge...
- SAI-infusion
- ASI-instruments
-
PALL仪器耗材-Pall Ca...
- 细胞目录-Cell Lines
- 美国 Cell Marque
- Hamilton
- 菌株 菌种库 微生物菌种
-
NU-CHEK
- ATCC细胞 细胞株 肿瘤细胞
- 丹麦NUNC全目录
CRL-1848 NCI-H292 人肺癌细胞(**结转移)
ATCC® Number: CRL-1848™
Designations: NCI-H292 [H292]
Depositors: AF Gazdar
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: lung
Disease: mucoepidermoid pulmonary carcinoma
Cellular Products: keratin; vimentin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility: Hepatitis B virus
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 9,13
D5S818: 13
D7S820: 10
THO1: 8
TPOX: 8,11
vWA: 16,17
Cytogenetic Analysis: This is a human cell line with near-diploid chromosome counts. The modal chromosome number was 47, occurring in 36% of cells. The rate of cells with a higher ploidy count was 3.9%. Twelve markers were common to most cells. Among them were del(1) (q32.1), der (5)t(5;13) (p15.33;q11), i(5p), der(1)t(1;?) (p34.3;?) and der (6)t(6;7) (p25.3;q21.2). All markers were present in single copy per cell. Normal N1 and N6 were absent. There were two normal X chromosomes. No other abnormalities were detected.
Age: 32 years
Gender: female
Ethnicity: Black
Comments: This line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma.
The cells were isolated in a chemically defined medium (HITES) and later adapted to growth in media supplemented with serum.
The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation.
The cells support the growth of hepatitis B virus and are negative for L-DOPA decarboxylase.
The line has been selected as a prototype for transfecting human subgenomic fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer.
The cells stain positive for keratin and vimentin and are mucicarmine positive but are negative for neurofilament triplet protein.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.