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  • 产品名称：CRL-8286 Reh 人急性非B非T**细胞白血病

  • 产品型号：CRL-8286
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简单介绍 CRL-8286 Reh 人急性非B非T**细胞白血病,原代细胞|细胞系|细胞株|菌种;细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件!
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CRL-8286 Reh 人急性非B非T**细胞白血病 的详细介绍

ATCC® Number: CRL-8286™    Price:
Designations: Reh
Depositors:  Univ. Pennsylvania
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Disease: acute lymphocytic leukemia (non-T; non-B)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Antigen Expression: CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%)
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: 95% (v/v) FBS; 5% (v/v) DMSO
Storage temperature: Liquid nitrogen vapor phase
References: 22700: Greaves M, Janossy G. Patterns of gene expression and the cellular origins of human leukaemias. Biochim. Biophys. Acta 516: 193-230, 1978. PubMed: 365240

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