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  • 产品名称：CRL-1472 HT-1376 人膀胱癌细胞

  • 产品型号：CRL-1472
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简单介绍 CRL-1472 HT-1376 人膀胱癌细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
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CRL-1472 HT-1376 人膀胱癌细胞 的详细介绍
CRL-1472 HT-1376 人膀胱癌细胞
ATCC® Number: CRL-1472™     Price: $323.00
Designations: HT-1376
Depositors:  S Rasheed
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: urinary bladder 
Tumor Stage: grade 3 
Disease: carcinoma
Cellular Products: fibrinolytic activity; interferon
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X 
CSF1PO: 12 
D13S317: 9,11 
D16S539: 11,14 
D5S818: 11,12 
D7S820: 9,12 
THO1: 7,10 
TPOX: 8 
vWA: 15,18
Cytogenetic Analysis: modal chromosome number = 104 to 121
Isoenzymes: G6PD, B
Age: 58 years
Gender: female
Ethnicity: Caucasian
Comments: The line does not contain detectable virus particles, herpesvirus antigens or retrovirus antigens. 
The cells will grow in soft agar.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended 
Medium Renewal: Every 2 to 3 days 
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow culture to sit at room temperature or 37C until cells detach (about 10 minutes), add fresh medium, aspirate and dispense into new flasks.
Related Products: purified DNA:ATCC CRL-1472D
References: 22538: Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628
23134: Jameson P, Grossberg SE. Production of interferon by human tumor cell lines. Arch. Virol. 62: 209-219, 1979. PubMed: 229805
25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484

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