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  • 产品名称：CV-1 非洲绿猴肾细胞

  • 产品型号：CCL-70 CV-1
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简单介绍 CV-1 非洲绿猴肾细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
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CV-1 非洲绿猴肾细胞 的详细介绍
CV-1 非洲绿猴肾细胞
ATCC® Number: CCL-70™    Price:
Designations: CV-1
Depositors:  FC Jensen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cercopithecus aethiops
Morphology: fibroblast
Source: Organ: kidney
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: efficacy testing [92587]
transfection host (Roche FuGENE® Transfection Reagents)
viruscide testing [92582] [92579]
Virus Susceptibility: poliovirus 1; herpes simplex; Eastern equine encephalitis; Western equine encephalitis; California encephalitis; SV40
Reverse Transcript: negative
Cytogenetic Analysis: This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell.
Age: 141 days
Gender: male
Comments: Suitable host for transfection, especially by SV40 vectors.
The CV-1 cell line was derived from the kidney of a male ***** African green monkey by F.C. Jensen, et al. in March, 1964 for use in Rous sarcoma virus transformation studies. [22138]
Derived from the kidney of male ***** African green monkey.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%


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