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  • 产品名称：CCL-233 SW1116 人结肠腺癌细胞

  • 产品型号：CCL-233
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简单介绍 CCL-233 SW1116 人结肠腺癌细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
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CCL-233 SW1116 人结肠腺癌细胞 的详细介绍
CCL-233 SW1116 人结肠腺癌细胞
ATCC® Number: CCL-233™     
Designations: SW1116 [SW 1116, SW-1116]
Depositors:  A Leibovitz
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
   
   
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Cytogenetic Analysis: modal number = 60; range = 50 to 62
The stemline chromosome number is hypotriploid with 2S component occurring at 2.8% and 9 markers were common to S metaphases. Neither HSR chromosomes nor DM were seen. Karyotypes were less variable between cells.
   
   
   
Ethnicity: Caucasian
Comments: CSAp negative (CSAp-).
Colon antigen 3, negative.
The cells are positive for keratin by immunoperoxidase staining.
The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes.
N-myc and N-ras oncogene expression were not detected.
Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Cells must be subcultured at about 80% confuency , before they reach 90%.
Protocol: 1. Remove and discard culture medium.

2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5. Add appropriate aliquots of the cell suspension to new culture vessels.

6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 1 to 2 times per week
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