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  • 产品名称：HTB-56 Calu-6 人肺癌细胞系（未分化癌）

  • 产品型号：HTB-56
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简单介绍 HTB-56 Calu-6 人肺癌细胞系（未分化癌）原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
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HTB-56 Calu-6 人肺癌细胞系（未分化癌） 的详细介绍
HTB-56 Calu-6 人肺癌细胞系（未分化癌）
ATCC® Number: HTB-56™     Price:  
Designations: Calu-6
Depositors:  J Fogh
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: unknown, probably lung 
Disease: anaplastic carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37C.

Interval: every 6 to 8 days 
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended 
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase

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