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  • 产品名称：D341 Med 人髓母细胞瘤

  • 产品型号：HTB-187
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D341 Med 人髓母细胞瘤
ATCC® Number: HTB-187™     Price:  
Designations: D341 Med
Depositors:  HS Friedman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: spheroid
D341 Med 人髓母细胞瘤
Source: Organ: brain 
Tissue: cerebellum 
Disease: medulloblastoma
Cellular Products: neurofilament proteins
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X,Y 
CSF1PO: 10,11 
D13S317: 11,13 
D16S539: 12,14 
D5S818: 11,12 
D7S820: 9,13 
F13A01: 6,7 
F13B: 8,10 
FESFPS: 10,13 
LPL: 11 
THO1: 6,9.3 
TPOX: 8,11 
vWA: 17,18
Cytogenetic Analysis: This is a hyperdiploid (modal number = 49; range = 44 to 50) human cell line with higher ploidies occurring with a frequency of 8.5%. Four marker chromosomes are present in all cells. They are: 11HSR, i(17q), der(22)t(1;22)(q12;p12) and 8q-. There are three copies each of N6 and N18, and single copies each of N11, N17 and N22. The single X chromosome was structurally normal, and the presence of the Y chromosome was confirmed by fluorescence microsopy.
Age: 3.5 years
Gender: male
Comments: The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma. 
The cells produce tumors in nude mice. 
The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. 
The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody. 
The c-myc oncogene is amplified in D341 Med cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 10(5) viable cells/ml. Maintain cultures between 4 X 10(5) and 1 X 10(6) cells/ml. 
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 90%; DMSO, 10% 
Storage temperature: liquid nitrogen vapor phase

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