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  • 产品名称：CRL-2632 Pfeiffer 人弥漫性大细胞**瘤B**细胞

  • 产品型号：CRL-2632
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简单介绍 CRL-2632 Pfeiffer 人弥漫性大细胞**瘤B**细胞，ATCC 细胞|细胞系|细胞株|细胞,细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件
详情介绍：
CRL-2632 Pfeiffer 人弥漫性大细胞**瘤B**细胞 的详细介绍
CRL-2632 Pfeiffer 人弥漫性大细胞**瘤B**细胞
ATCC® Number: CRL-2632™     Price:  
Designations: Pfeiffer
Depositors:  H Ben-Bassat
Isotype: IgG kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma 
Derived from metastatic site: pleural effusion 
Cell Type: lymphoblast B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Isolation: Isolation date: 1992
Antigen Expression: CD10; Homo sapiens 
CD19; Homo sapiens 
CD20; Homo sapiens 
CD38; Homo sapiens 
CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289]
DNA Profile (STR): CSF1PO: 10, 11 
D13S317: 11, 12 
D16S539: 12 
D5S818: 10, 13 
D7S820: 8, 12 
THO1: 9, 9.3 
TPOX: 9 
vWA: 17, 18 
Amelogenin: XY
Cytogenetic Analysis: The cell line contains several chromosomal abberations including the typical t(14;18)(q32;q21) translocation of follicular lymphomas.
Age: ***** *****
Gender: male
Ethnicity: White
Comments: The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei. [53289] 
The cells are negative for Epstein-Barr virus. [53289] 
This cell line is a model system for studying non-Hodgkin lymphomas.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Doubling Time: 24 to 30 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
ATCC® Number: CRL-2632™ Price: $379.00 
Designations: Pfeiffer 
Depositors: H Ben-Bassat 
Isotype: IgG kappa 
Biosafety Level: 1 
Shipped: frozen 
Medium & Serum: See Propagation 
Growth Properties: suspension 
Organism: Homo sapiens (human) 
Morphology: lymphoblast


Source: Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma 
Derived from metastatic site: pleural effusion 
Cell Type: lymphoblast B lymphocyte; 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 

Isolation: Isolation date: 1992 
Antigen Expression: CD10; Homo sapiens 
CD19; Homo sapiens 
CD20; Homo sapiens 
CD38; Homo sapiens 
CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289] 
DNA Profile (STR): CSF1PO: 10, 11 
D13S317: 11, 12 
D16S539: 12 
D5S818: 10, 13 
D7S820: 8, 12 
THO1: 9, 9.3 
TPOX: 9 
vWA: 17, 18 
Amelogenin: XY 
Cytogenetic Analysis: The cell line contains several chromosomal abberations including the typical t(14;18)(q32;q21) translocation of follicular lymphomas. 
Age: ***** ***** 
Gender: male 
Ethnicity: White 
Comments: The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei. [53289] 
The cells are negative for Epstein-Barr virus. [53289] 
This cell line is a model system for studying non-Hodgkin lymphomas. 
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) 
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5% 
Doubling Time: 24 to 30 hrs 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020 
References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 

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