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  • 产品名称：HTB-40 HuTu-80 人十二指肠腺癌细胞

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简单介绍 HTB-40 HuTu-80 人十二指肠腺癌细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
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HTB-40 HuTu-80 人十二指肠腺癌细胞 的详细介绍
HTB-40 HuTu-80 人十二指肠腺癌细胞
ATCC® Number: HTB-40™     Price:  
Designations: HuTu 80
Depositors:  V Larson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: duodenum 
Disease: adenocarcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Receptors: bombesin
Tumorigenic: Yes
Antigen Expression: Blood Type B; Rh+
DNA Profile (STR): Amelogenin: X,Y 
CSF1PO: 11,13 
D13S317: 8,11 
D16S539: 10,11 
D5S818: 12,13 
D7S820: 9,11 
THO1: 7 
TPOX: 9,11 
vWA: 16,18
Cytogenetic Analysis: modal number = 46; range = 42 to 48.
This is a pseudodiploid human cell line. The modal chromosome number is 46 occurring at 68% and polyploidy at 0.4%. Three marker chromosomes were detected in all metaphases examined. Two markers that appeared to be the product of the balanced translocation between N1 and N17. The third marker is ?inv(13). No other common markers were detected. Normal N1, N13, and N17 had single-copy each. Y chromosome is present.
Isoenzymes: AK-1, 1 
ES-D, 1 
G6PD, B 
GLO-I, 2 
Me-2, 2 
PGM1, 1-2 
PGM3, 1-2
Age: 53 years
Gender: male
Ethnicity: Caucasian
Comments: The cells express receptors for bombesin at up to 6000 sites per cell.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended 
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
 

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