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  • 产品名称： HTB-10 SK-N-MC 人神经上皮瘤细胞

  • 产品型号： HTB-10
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简单介绍 HTB-10 SK-N-MC 人神经上皮瘤细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
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HTB-10 SK-N-MC 人神经上皮瘤细胞 的详细介绍
HTB-10 SK-N-MC 人神经上皮瘤细胞
ATCC® Number: HTB-10™     Price:  
Designations: SK-N-MC
Depositors:  G Trempe, LJ Old
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: brain 
Disease: neuroepithelioma 
Derived from metastatic site: supra-orbital area
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes
Antigen Expression: Blood Type O; Rh+
DNA Profile (STR): Amelogenin: X 
CSF1PO: 10 
D13S317: 11 
D16S539: 12 
D5S818: 11 
D7S820: 8 
THO1: 9.3 
TPOX: 9,11 
vWA: 17,18
Cytogenetic Analysis: The cell line is a pseudodiploid human female (XX), with chromosome counts in the diploid range and a modal chromosome number of 46. Normal chromosomes N3 and N10 are absent, and many (N1, N2, N4, N15, N16, N17, N21, and N22) are monosomic. Normal chromosome N8 is most often tetrasomic. The remainder of normal chromosomes were usually paired. Numerous marker chromosomes are present including: 1p+, der(3)t(2;3)(q24;q27), del(4)(p12), 11q+, del(2)(q23), ampl.(17)(p12), ampl.(16)(q13), del(15)(q13q22), 21p+, iso(3q), del(22)(q11q13). Marker chromosomes M2 and M3 appear to us to be identical to two markers (M4 and M3, respectively) described by R.C. Seeger, et al.for this cell line. [26318]
Isoenzymes: AK-1, 1 
ES-D, 2 
G6PD, B 
GLO-I, 1-2 
Me-2, 2 
PGM1, 1 
PGM3, 1-2
Age: 14 years
Gender: female
Ethnicity: Caucasian
Comments: This is one of two cell lines (see ATCC HTB-11) of neurogenic origin derived by J.L. Biedler.SK-N-MC was isolated in September of l971 and was found to have moderate dopamine - beta - hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C
Subculturing:  
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase
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