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  • 产品名称：CCL-56 Pt-K2 袋鼠肾细胞

  • 产品型号：CCL-56
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简单介绍 CCL-56 Pt-K2 袋鼠肾细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
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CCL-56 Pt-K2 袋鼠肾细胞 的详细介绍
CCL-56 Pt-K2 袋鼠肾细胞
ATCC® Number: CCL-56™    Price:
Designations: PtK2 (NBL-5)
Depositors:  KH Whalen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Potorous tridactylis (potoroo)
Morphology: epithelial
Source: Organ: kidney
Disease: normal
Cellular Products: keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Virus Resistance: adenovirus 5; coxsackievirus B5; poliovirus 2
Age: *****
Gender: male
Comments: The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 daysEvery 2 to 3 days
Preservation: Freeze medium: growth medium, 95%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 26208: . . Genetics 51: 915, 1965.


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