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  • 产品名称：TIB-18 P3/NSI/1-Ag4-1[NS-1] 小鼠骨髓瘤细胞

  • 产品型号：TIB-18
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简单介绍 TIB-18 P3/NSI/1-Ag4-1[NS-1] 小鼠骨髓瘤细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
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TIB-18 P3/NSI/1-Ag4-1[NS-1] 小鼠骨髓瘤细胞 的详细介绍
TIB-18 P3/NSI/1-Ag4-1[NS-1] 小鼠骨髓瘤细胞
ATCC® Number: TIB-18™    Price:
Designations: P3/NSI/1-Ag4-1 [NS-1]
Depositors:  G Kohler, C Milstein
Isotype: kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (mouse)
Morphology: lymphoblast
TIB-18 P3/NSI/1-Ag4-1[NS-1] 小鼠骨髓瘤细胞
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin (not secreted)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Antigen Expression: H-2d
Comments: This is a non-secreting clone of P3X63Ag8 (ATCC TIB-9).
Kappa chains are synthesized but not secreted.
The cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium.
The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Do not allow the cell density to exceed 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liqid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
parental cell line:ATCC TIB-9
References: 956: Barnstable CJ, et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens -- new tools for genetic analysis. Cell 14: 9-20, 1978. PubMed: 667938
1115: Kohler G, et al. Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines. Eur. J. Immunol. 6: 292-295, 1976. PubMed: 825374
22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
22347: Kohler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511-519, 1976. PubMed: 825377
23366: Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588
26333: Ramasamy R, et al. Possible role for the Fc receptor on B lymphocytes. Nature 249: 573-574, 1974. PubMed: 4545851
26344: Cowan NJ, et al. Intracellular immunoglobulin chain synthesis in non-secreting variants of a mouse myeloma: detection of inactive light-chain messenger RNA. J. Mol. Biol. 90: 691-701, 1974. PubMed: 4449137
33092: Condie R, et al. Cannabinoid inhibition of adenylate cyclase-mediated signal transduction and interleukin 2 (IL-2) expression in the murine T-cell line, EL4.IL-2. J. Biol. Chem. 271: 13175-13183, 1996. PubMed: 8662742


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