- 美国DRIERITE目录-DRI...
- 美国Nationaldiagno...
- 美国Electron Micro...
-
美国电镜耗材总目录
-
脑矩阵
-
标样
-
碳/石墨棒
- 清洁用品
-
钻石刀
-
TEM支持膜
- 胶黏剂非导电 胶黏剂导电
-
烧杯
-
原子力显微镜用品
-
AFM探针
- 防静电产品
- 胶黏剂产品
-
铜网、镍网和金网
- Adhesives 胶粘剂 粘合...
-
Apertures 光圈孔径
-
Atomic Force Mic...
-
Beakers 烧杯
-
Brushes 生物刷
-
Calibration 校准产品
-
Carbon / Graphit...
-
Cleaning 清洁产品
-
Dissection Suppl...
-
Filaments / Cath...
-
Embedding Suppli...
-
Forensic 法医产品
-
FIB Supplies 纤维...
-
Grids 电镜网格系列
-
Materials Scienc...
-
Omniprobe Consum...
-
Scanning Electro...
-
Tweezers 镊子
-
Tools 工具
-
脑矩阵
-
KOVA公司产品
- 美国EIS
- 美国Tronex
-
APPLIED MICROARR...
- Drummondsci
- 美国PrimCells公司
- 美国Vector目录-Vecto...
-
美国VWR目录-VWR Cata...
-
General Catalogu...
- Adhesives-Autora...
- Bags-Barometers ...
- Baths-Blenders 水...
- Bottles 试验瓶
- Brushes-Casserol...
- Centrifuges-Chlo...
- Chromatography 层...
- Clamps-Cylinders...
- Data Loggers-Ele...
- Electrophoresis ...
- Electroporators-...
- Filters-Flashlig...
- Flasks-Furnaces ...
- Gas-Heating Tape...
- Hemacytometers-H...
- Humidity-Ice Mak...
- Incubators-Melti...
- Microbiology Med...
- Microscopes-Mill...
- Molecular Biolog...
- Ovens-Petroleum ...
- pH-Photometers 酸...
- Pipets-Pipettors...
- Plates-Rulers 酶标...
- Safety, Air Moni...
- Safety, Hearing ...
- Sample Cups-Spec...
- Spectrophotomete...
- Standards 标准液
- Stationery-Stopp...
- Surface Tension-...
- Thermometers-Tim...
- Tissue-Tubes,NMR...
- Tubing-Valves 导管...
- Vials-Watch Glas...
- Water Purificati...
-
Chemical Catalog...
- Acacia to Alumin...
- Amine-Sulfuric A...
- Balsam Canada So...
- Cacodylic Acid, ...
- Deblock Reagent ...
- Ecolite to Furfu...
- D-(+)-Galactose ...
- Imidazole to Lys...
- Magnesium to Myr...
- Naphthalene to 2...
- Palladium (II) A...
- Potassium Acetat...
- Saccharin Sodium...
- Sorbic Acid to S...
- TAE Buffer to Tr...
- Universal Indica...
-
Life Science Cat...
- Air-Antibodies 压...
- Apoptosis Reagen...
- Autoradiography-...
- Blotting-Bottles...
- Cell Cycle Reage...
- Centrifuges-Comb...
- Competent Cells ...
- Conductivity-Dia...
- DNA Synthesis Re...
- Electrophoresis-...
- Enzymes 酶
- Evaporators-Free...
- Fume Hoods-Histo...
- Homogenizers-Loo...
- Microscopes/Slid...
- Molecular Biolog...
- Nitric Oxide Rea...
- Peptide Synthesi...
- Petri-Photodocum...
- Pipetting 吸液管
- Plate Readers-Pl...
- Reagents, Specia...
- Refrigerators-Th...
- Tissue-Wipers 组织...
-
General Catalogu...
- 美国MatTek Corpora...
-
美国Nasco Whirl-Pa...
-
Whirl-Pak圆底采样袋
-
Whirl-pak耐高温采样袋
-
Whirl-Pak大肠菌群采样袋
-
Whirl-Pak均质袋
-
Whirl-Pak黑色采样袋
-
Whirl-Pak无菌采样袋(附...
-
Whirl-Pak可放置标牌的无...
-
Whirl-Pak防辐射采样袋
-
Whirl-Pak带泡沫采样袋
-
Whirl-Pak长期保存样品袋
-
Trans-Pak取样袋,带生物...
-
Whirl-Pak可标记宽钢丝封...
-
Whirl-Pak直立含硫代硫酸...
-
Whirl-Pak大容积采样袋
-
Whirl-Pak均质过滤袋
-
Whirl-Pak表面测试采样袋...
-
Whirl-Pak无菌水质采样袋
-
Whirl-Pak可直立圆底采样...
-
Whirl-Pak带标签无菌采样...
-
Whirl-Pak标准无菌采样袋
- Whirl-Pak®Bags 美...
-
Whirl-Pak圆底采样袋
- Berkshire
- 美国CORNING目录-CORN...
-
美国(Thermo Fisher...
-
**组化笔—PAP PEN
-
Thermo玻片-Thermo ...
- Fisher玻片-Fisher ...
- VWR玻片-VWR Slide
-
Corning玻片
-
NUNC玻片目录- NUNC C...
-
紫外光谱相关-UV spectr...
-
HAMILTON进样器-HAMI...
-
计时器-Timer
-
游标式瓶口分配器-Cursor-...
-
AMPULES安瓿瓶-Ampou...
-
袋子-Bags
-
称量用品-Weighing Pr...
-
烧杯-Beaker
-
实验台保护垫-Test bed ...
-
试剂瓶-Reagent bott...
-
滴定管-Buret
-
游标卡尺-Calipers
-
试剂桶-Reagent barr...
-
夹子和台架-Clip and S...
-
储样-Sample storag...
-
坩埚-Crucible
-
冻存-Cryopreservat...
-
量筒-Graduated cyl...
-
平皿-Plate
-
过滤产品-Filtration ...
-
烧瓶-Flask
-
容量瓶-Volumetric f...
-
地粘-stick
-
镊子-Forceps
-
漏斗-Funnel
-
玻璃割刀-Glass cutte...
-
手套-Gloves
-
组织学用具-Histology ...
-
冰盒-Ice box
-
标签带-Label with
-
微生物取样-Microbial ...
-
微量板-Micro plate
-
显微镜耗材-Microscope...
-
玛瑙研钵-Agate morta...
-
支架-Support
-
**用品-Safety Prod...
-
取样器-Sampler
-
剪刀-Scissors
-
标准筛-Standard sie...
-
抹刀-Spatula
-
磁力搅拌子-Magnetic s...
-
胶塞-Stopper
-
组织培养-Tissue Cult...
-
夹子-Clip
-
分配盘/托盘-Distribut...
-
管子-Tube
-
样品小瓶-Sample vial
-
利器存储桶-Tool bucke...
-
实验室用抹布-Laborator...
-
封口膜-Sealing film
-
移液管及**装置-Pipette...
-
移液枪-Pipette
-
**组化笔—PAP PEN
- 美国BD 公司产品
- 美国Nalgene 生物耗材
- **新品-New Product...
- VWR目录-VWR Catalo...
-
Corning目录-Cornin...
-
Millipore仪器耗材-Mi...
-
Merck试剂耗材-Merck ...
-
Whatman试剂耗材-What...
-
Axygen试剂耗材-Axyge...
- SAI-infusion
- ASI-instruments
-
PALL仪器耗材-Pall Ca...
- 细胞目录-Cell Lines
- 美国 Cell Marque
- Hamilton
- 菌株 菌种库 微生物菌种
-
NU-CHEK
- ATCC细胞 细胞株 肿瘤细胞
- 丹麦NUNC全目录
HTB-128 MDA-MB-415 人乳腺腺癌细胞
ATCC® Number: HTB-128™
Designations: MDA-MB-415
Depositors: R Cailleau
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: mammary gland; breast
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1975
Tumorigenic: No
Antigen Expression: Blood Type O
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 11,13
D16S539: 13
D5S818: 11,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 17
Cytogenetic Analysis: modal number =73; range = 65 to 76. The stemline chromosome number is hypertriploid with the 2S component occurring at 0.8%. Ten markers (del(2), t(6;?), t(6;?), t(2;12), t(14;?), and single or paired M6, M7, M8, M9, and M10) were common to all cells, and four others (M13, M15, M16, and t(13,21) occurred in some cells. The X chromosome was generally tetrasomic and chromosome 4 was tetrasomic or pentasomic (P35). No Y chromosomes were detected in QM stained preparations.
Isoenzymes: AK-1, 1
ES-D, 2
G6PD, A
GLO-I, 1-2
Me-2, 2
PGM1, 1
PGM3, 1
Age: 38 years *****
Gender: female
Comments: The cells express the WNT7B oncogene [PubMed: 8168088].
The patient presenting with the tumor was from Paraguay and, although listed as Caucasian, may have had mixed ancestry as suggested by the presence of G6PD type A phenotype in the cells.
The line forms flat, spreading plaques of epithelia which exhibit desmosomes, extensive microtubules and microfilaments upon examination with the electron microscope. It is not amenable to trypsinization.
Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 2mM L-glutamine supplemented with 10 mcg/ml insulin and 10 mcg/ml glutathione, 85%; fetal bovine serum, 15%
Atmosphere: air, ****
Temperature: 37.0°C
Subculturing: Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
purified DNA:ATCC 45524
purified DNA:ATCC 45525
purified DNA:ATCC HTB-128D
References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337
22329: Beall PT, et al. Polypeptides that inhibit human breast cancer cell division. Cancer Biochem. Biophys. 3: 93-96, 1979. PubMed: 552902
22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779
22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202
23113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088