产品名称：CRL-2021 MEG-01 人成巨核细胞白血病细胞

CRL-2021 MEG-01 人成巨核细胞白血病细胞
ATCC® Number:  CRL-2021™      
Designations:  MEG-01 
Depositors:   M Tekeuchi, MJ Fitzgerald 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: mixed, adherent and suspension
Organism: Homo sapiens (human) 
Morphology: lymphoblast

 
Source: Disease: chronic myelogenous leukemia (CML)
Cell Type: megakaryoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Applications: transfection host (Nucleofection technology from Lonza)
Antigen Expression: CD41 +; CD61 +; CDw14 +
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 8
D16S539: 9
D5S818: 13
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16
Cytogenetic Analysis: hyperdiploid; modal number = 56 to 58; the Philadelphia chromosome (Ph1) is present
Age:  55 years 
Gender:  male 
Comments: The MEG-01 cell line was derived in 1983 at the Nagoya University School of Medicine, Nagoya, Japan from bone marrow cells taken from a patient in megakaryoblastic crisis of CML.
The cells are positive for cytoplasmic Factor VIII and surface GPIIb/IIIa, periodic acid - Schiff (PAS) reaction, alpha naphthyl acetate esterase and acid phosphatase.
They are negative for myeloperoxidase, alpha naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase and alkaline phosphatase.
The stain positively with monoclonal antibodies BA-1 (anti B cell, granulocyte), HPL-3 (anti gpIIb/IIIa) and 20.3 (anti monocyte, platelet).
They are negative for other lymphoid and myeloid seris antigens.
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:  Protocol: Subcultures are prepared by scraping the adherent cells into the medium. From the resulting suspension dilute cells to a concentration 1-2 X 10exp5 cells/ml into fresh medium in a new flasks. Keep culture below approximate density of 10exp6 cells/ml.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures are prepared by scraping the adherent cells into the medium, and diluting the resulting suspension into fresh medium in new flasks.
Doubling Time:  36 to 48 hrs 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 23348: Ogura M, et al. Establishment of a novel human megakaryoblastic leukemia cell line, MEG- 01, with positive Philadelphia chromosome. Blood 66: 1384-1392, 1985. PubMed: 2998511