产品详情
查看大图

  • 产品名称：HTB-107 SW579 人甲状腺鳞癌细胞

  • 产品型号：HTB-107
  • 产品厂商：其它品牌
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
简单介绍 HTB-107 SW579 人甲状腺鳞癌细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
详情介绍：

HTB-107 SW579 人甲状腺鳞癌细胞 的详细介绍
HTB-107 SW579 人甲状腺鳞癌细胞
ATCC® Number: HTB-107™     Price:  
Designations: SW579 [SW 579, SW-579]
Depositors:  A Leibovitz
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: thyroid 
Disease: squamous cell carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Isolation: Isolation date: 1973
Tumorigenic: Yes
Antigen Expression: blood type O; Rh+
DNA Profile (STR): Amelogenin: X 
CSF1PO: 13 
D13S317: 13 
D16S539: 11 
D5S818: 11 
D7S820: 8,9 
THO1: 8,9.3 
TPOX: 8,10 
vWA: 14,18
Isoenzymes: AK-1, 1 
ES-D, 1 
G6PD, B 
GLO-I, 2 
Me-2, 1-2 
PGM1, 1-2 
PGM3, 1
Age: 59 years
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, **** 
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended 
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

沪公网安备 31010902002433号