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*声波振动钻石刀Ultra Sonic Oscillating Diamond Knife
*声波振动钻石刀是室温薄切片的选择,也是近些年来DiATOME与Dr Daniel Studer,(Lab. of Anatomy, University of Berne)合作的结晶。*声波振动切片是以*声频振动激励钻石刀以帮助改善切片性能方法,这种切片方法的研究,近年在美、日、苏、西德、英等国取得较大进展并逐渐得到应用。其具有减少切力、提高加工精度、提高表面光洁度和表面质量、延长刀具寿命等。Ultra Sonic Oscillating Diamond Knife钻石刀彻底解决了切片过程中的“样本压缩”问题。通常在切片过程中产生“样本压缩”的原因在于:
进刀的角度
样品的硬度
钻石表面和样品表面的相互作用
切片的厚度
钻石刀以固定的频率和振幅来回运动,振荡的方向要平行于刀锋。在室温下*声振荡钻石刀制备的样品
消除了压缩的想象,让使用者可以得到薄,质量好的切片。
我们已经进行以下包埋样品的测试,其结果都非常理想:
生物学样品in Epon, Araldite, EM Bed, etc
生物学样品in acrylic resins (Lowicryls, LR White)
硬的聚合材料PS, PMMA, ABS, HIPS, modified PP, etc
该款钻石刀的参数:
35º
刀锋长3.0mm
切片厚度10-80nm
控制机单元 Control Unit
频率25-45kHz,或者自动共振装置
振幅可变(电压0-30V)
机器电压230V,110V
货号 |
品名 |
角度 |
长度 |
30-ULTSON |
UltraSonic Knife and Control Unit |
35° |
3.0mm |
30-ULTSONK |
UltraSonic Knife |
35° |
3.0mm |
一些使用的参考文献:
A. Al-Amoudi, J.Dubochet, H. Gnaegi, W. Lüthi, D.Studer: An oscillating cryo-knife reduces cuttinginduced deformation of vitreous ultrathin sections. Journal of Microscopy, Vol. 212, Pt 1, pp. 26-33, 2003.
D. Studer and H. Gnägi: Minimal compression of ultrathin sections with use of an oscillating diamond knife. Journal of Microscopy, Vol. 197, Pt 1, pp. 94-100, 2000.
J.S. Vastenhout and H.Gnaegi: Ultramicrotomy of polymers using an oscillating knife; improving polymer morphology. Microscopy and Microanalysis. 8 (Suppl. 2) 2002
使用此款钻石刀得到的杰出作品展示如下:
Peripheral nerve (rat)
Peripheral nerv (rat), HP frozen, freeze substituted, Epon embedded, cut with the ultra sonic knife, section thickness 50nm. W. Graber, Institut of Anatomy, University of Bern, Switzerland
ABS, stained with OsO4, sectioned at room temperature with the ultra sonic knife, section thickness 50nm. Note the almost perfect spherical shape of the large rubber particles and the preservation of the inclusions inside. Also the smaller dense rubber particles are well preserved. B.Vastenhout, Dow Benelux N.V. Terneuzen, The Netherlands.
Heart (rat) |
Heart (rat), fixed with 2.5% Glutaraldehyde, OsO4 stained, Spurr's embedded, cut with the ultra sonic knife, section thickness setting 15nm. J. Nebesarova, Institut of Parasitology, Ceske Budejovice, Czech Republic. Imaged by Petr Stepan in LVEM 5 at 5kV, Delong Instruments, Brno, Czech Republic.