产品名称：VECTASHIELD Mounting Medium with DAPI 封片剂

VECTASHIELD® Mounting Medium with DAPI 
Catalog No. H-1200 
Amount 10 ml 
Concentration of DAPI 1.5 µg/ml 
Storage Conditions Refrigerate in the dark 
VECTASHIELD® Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD® 
Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes (see website for 
compatibility). 
This special formulation of VECTASHIELD® Mounting Medium contains 4’,6-diamidino-2-phenylindole (DAPI), which 
fluoresces when bound to DNA and is used as a nuclear or chromosomal counterstain. This product is designed 
to be used for in situ hybridization techniques or other procedures requiring fluorescent labeling of DNA. DAPI 
may also stain RNA but displays greater selectivity for binding DNA over RNA. DAPI excites at about 360 nm and 
emits at about 460 nm when bound to DNA, producing a blue fluorescence. 
VECTASHIELD® Mounting Medium is supplied in a bottle fitted with a screw cap. A cap fitted with a drop 
dispenser pipet is also included. The screw cap can be replaced with the drop dispenser cap for routine use and 
during storage. Once the drop dispenser cap has been secured on the bottle, the bottle should be stored in an 
upright position. 
To mount tissues or cells on a slide, dispense VECTASHIELD® Mounting Medium onto the specimen. The special 
pipet supplied with the product is designed to deliver small drop volumes of approximately 25 µl. Coverslip and 
allow VECTASHIELD® Mounting Medium to disperse over the entire specimen. 
VECTASHIELD® Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without 
sealing. If desired, coverslips can be permanently sealed around the perimeter with nail polish or a plastic 
sealant. Mounted slides should be stored at 4 °C protected from light.