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  • 产品名称：Hydrogen Peroxide Assay Kit过氧化氢检测试剂盒

  • 产品型号：CL-204
  • 产品厂商：其它品牌
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简单介绍：
Hydrogen Peroxide Assay Kit 过氧化氢检测试剂盒
详情介绍：

 Hydrogen Peroxide Assay Kit
过氧化氢检测试剂盒 
   

  • Sensitive, Quantitative Assay for Hydrogen Peroxide
  • Detects as Little as 15ng/ml
  • Easy-to-use, Colorimetric System
Catalog Number:CL-204

 

1.灵敏的定量测定过氧化氢

2.*低检测限为15ng/ml

3.采用比色方法，使用简便

过氧化氢是氧还原途径的产物，由分子氧双电子还原或者超氧阴离子自由基单电子还原产生。过氧化氢为强氧化剂，其浓度水平必须准确检测以完全确认被研究系统的氧化状态。ND的过氧化氢检测试剂盒提供了快速，灵敏，定量的检测化学或生物系统中过氧化氢水平的方法。检测方法基于二甲酚橙和三价铁形成的复合物显色反应，而三价铁由过氧化物氧化二价铁得到。反应可以比色定量化，过氧化物的*低检测限为15ng/ml。该试剂盒是Diagenes过氧化物检测试剂盒的补充，通过先后使用两个试剂盒，研究者就可以定量检测氧还原途径的前两步反应。

 


Hydrogen peroxide is a part of the oxygen reduction pathway, produced by the two-electron reduction of molecular oxygen, or by the one electron reduction of superoxide anion radical. Hydrogen peroxide is a potent oxidant, and the levels of hydrogen peroxide must be accurately determined in order to fully characterize the oxidative state of the system under study.

National Diagnostics Hydrogen Peroxide Assay Kit is a rapid, sensitive and quantitative method for the determination of Hydrogen Peroxide in chemical or biological systems. The assay is based upon formation of a complex between Xylenol Orange and ferric iron, which is produced by the peroxide dependent oxidation of ferrous iron. This reaction is quantified colorimetrically, detecting as little as 15ng/ml of peroxide.

National Diagnostics Hydrogen Peroxide Assay is the ideal complement to our well established Diogenes Superoxide detection system. Using these kits in tandem, researchers can now quantitatively determine the first two steps in the oxygen reduction pathway.


Hydrogen Peroxide Assay Protocol

Assay Reagent Preparation
Prepare 1.8ml of reagent per sample to run tests in duplicate.
1. To prepare 20ml: Combine 19.8ml of Component A with 0.2ml
Component B.
2. The mixture is stable at room temperature for one working day.

Assay Procedure
1. Mix 0.9ml of Assay Reagent with up to 0.1ml of sample.
2. Incubate at room temperature for at least 30 minutes to allow
for complete color development.
3. Read absorbance at 560nm.



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