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  • 产品名称:Diogenes 细胞化学发光增强试剂盒

  • 产品型号:CL-202
  • 产品厂商:其它品牌
  • 产品文档:
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简单介绍:
Diogenes 细胞化学发光增强试剂盒
详情介绍:

Diogenes 细胞化学发光增强试剂盒  

  • Cellular Luminescence Enhancement System for Superoxide Detection
  • Easy to Use - Specific for Superoxide
  • From 100-Fold to 600-Fold Enhancement
  • Requires Fewer Cells - Non Cytotoxic
Catalog Number:CL-202

 

1.适用于过氧化物检测的细胞发光增强剂

2.使用方便特别适用于过氧化物

3.增强信号100-600倍

4.只需要非常少的细胞且对细胞无毒性

Diogenes细胞化学发光增强系统是一种过氧化物化学发光增强剂不会使活细胞变性失活。*氧自由基会在细胞有氧呼吸或者白细胞抵御感染的过程中产生。白细胞在受到fMLP、佛波酯、抗Fc受体的抗体以及LPS刺激时进行氧化反应的程度可以部分作为**活力的指标。现在测定白细胞产生的*氧自由基的方法都非常的繁琐而且不直接,例如在氰化物存在或缺失的条件下测定克拉克氏电*对氧气的吸收或者测定细胞色素c被还原时发生的光谱改变。Diogene试剂盒利用生成无毒性中间产物的发光反应,非常适用于细胞介导的过氧化物生成检测。过氧化物氧化反应发射化学荧光的强弱与*氧自由基的浓度成正比,而且比使用鲁米诺发出的光强很多。因此Diogenes非常适合于检测细胞的**活性,只需要使用化学光度计便可定量光信号的强弱。任何刺激氧化酶产生胞外过氧化物的反应都可以用Diogenes检测。可以在生理条件下或者模仿生理条件下完成过氧化物的检测。

 

Diogenes Cellular Luminescence Enhancement System is a superoxide chemiluminescent enhancer that is non-denaturing to living cells. Superoxide radical (O2-) is produced intracellularly as a consequence of aerobic metabolism and extracellularly by leukocytes in response to infection. The extent of oxidative burst produced by white blood cells (WBCs) when stimulated by f-met-leu-phe, phorbol esters, anti-Fc receptor antibodies or LPS is a partial indicator of the immunocompetence of the cells tested.

Currently, the production of O2- by leukocytes is monitored by such cumbersome and indirect methods as measuring oxygen uptake in a Clark electrode (both in the presence and absence of cyanide) or measuring spectral changes caused by the reduction of cytochrome c. As a non-cytotoxic intermediate in the mechanism of photon production, Diogenes is ideally suited to the detection of cell-mediated superoxide production. The intensity of light produced by Diogenes in the presence of superoxide is directly proportional to the O2- concentration, but is much higher than that achieved by using luminol. Therefore, Diogenes is ideal for monitoring cellular immunocompetence, utilizing a luminometer to quantify the light output. Any stimulant that activates an oxidase to produce extracellular superoxide is usable with Diogenes. Such means can be physiological ormimetic of the physiologic pathway.


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