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  • 产品名称：CCL-88 Tb 1 Lu 蝙蝠肺细胞

  • 产品型号：CCL-88
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简单介绍：
简单介绍 CCL-88 Tb 1 Lu 蝙蝠肺细胞,原代细胞、细胞系。细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件，
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CCL-88 Tb 1 Lu 蝙蝠肺细胞 的详细介绍
CCL-88 Tb 1 Lu 蝙蝠肺细胞
ATCC® Number: CCL-88™    Price:
Designations: Tb 1 Lu
Depositors:  AJ Kniazeff, D Constantine, WA Nelson-Rees, D Schmidt
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Tadarida brasiliensis (bat, free-tailed)
Morphology: epithelial
Source: Organ: lung
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Isolation: Isolation date: October, 1965
Virus Resistance: adenovirus 5; coxsackievirus A9, B5; herpes simplex; bovine diarrhea; infectious bovine rhinotracheitis; poliovirus 2
Cytogenetic Analysis: modal number = 48; range = 42 to 53
Polyploidy about 1%. Dicentric chromosomes or chromosomes with chromatids fused at distal end in 6% of cells.
Age: *****
Gender: female
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor pahse
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020


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