产品详情
查看大图

  • 产品名称： NRK-49F 大鼠正常肾成纤维细胞

  • 产品型号：CRL-1570
  • 产品厂商：其它品牌
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
简单介绍 NRK-49F 大鼠正常肾成纤维细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和**培养条件！
详情介绍：

NRK-49F  大鼠正常肾成纤维细胞
ATCC® Number: CRL-1570™    Price:
Designations: NRK-49F
Depositors:  JE DeLarco
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus (rat)
Morphology: fibroblast
Source: Organ: kidney
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Receptors: epidermal growth factor (EGF); multiplication stimulating activity (MSA)
Comments: NRK-49F cells are contact inhibited and very sensitive to transformation by chemicals and viruses.
Cultures should be subcultured before they become confluent.
If allowed to become confluent, the cells will transform.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 22713: de Larco JE, Todaro GJ. Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation. J. Cell. Physiol. 94: 335-342, 1978. PubMed: 304450
23197: Huu D, et al. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92: 1133-1140, 1966. PubMed: 5926742
32442: Sachsenmeier KF, et al. Transforming growth factor-beta1 inhibits nucleosomal fragmentation in human keratinocytes following loss of adhesion. J. Biol. Chem. 271: 5-8, 1996. PubMed: 8550610
32705: Schultz-Cherry S, Hinshaw VS. Influenza virus neuraminidase activates latent transforming growth factor beta. J. Virol. 70: 8624-8629, 1996. PubMed: 8970987
33046: Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609
33159: Centrella M, et al. Rapid flux in transforming growth factor-beta receptors on bone cells. J. Biol. Chem. 271: 18616-18622, 1996. PubMed: 8702513


沪公网安备 31010902002433号